Smallpox vaccination induces a substantial increase in commensal skin bacteria that promote pathology and influence the host response.

Autor: Shmeleva EV; Department of Pathology, University of Cambridge, Cambridge, United Kingdom., Gomez de Agüero M; Maurice Müller Laboratories, Department for Biomedical Research, Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland., Wagner J; Parasites and Microbes, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, United Kingdom., Enright AJ; Department of Pathology, University of Cambridge, Cambridge, United Kingdom., Macpherson AJ; Maurice Müller Laboratories, Department for Biomedical Research, Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland., Ferguson BJ; Department of Pathology, University of Cambridge, Cambridge, United Kingdom., Smith GL; Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2022 Apr 21; Vol. 18 (4), pp. e1009854. Date of Electronic Publication: 2022 Apr 21 (Print Publication: 2022).
DOI: 10.1371/journal.ppat.1009854
Abstrakt: Interactions between pathogens, host microbiota and the immune system influence many physiological and pathological processes. In the 20th century, widespread dermal vaccination with vaccinia virus (VACV) led to the eradication of smallpox but how VACV interacts with the microbiota and whether this influences the efficacy of vaccination are largely unknown. Here we report that intradermal vaccination with VACV induces a large increase in the number of commensal bacteria in infected tissue, which enhance recruitment of inflammatory cells, promote tissue damage and influence the host response. Treatment of vaccinated specific-pathogen-free (SPF) mice with antibiotic, or infection of genetically-matched germ-free (GF) animals caused smaller lesions without alteration in virus titre. Tissue damage correlated with enhanced neutrophil and T cell infiltration and levels of pro-inflammatory tissue cytokines and chemokines. One month after vaccination, GF and both groups of SPF mice had equal numbers of VACV-specific CD8+ T cells and were protected from disease induced by VACV challenge, despite lower levels of VACV-neutralising antibodies observed in GF animals. Thus, skin microbiota may provide an adjuvant-like stimulus during vaccination with VACV and influence the host response to vaccination.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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