| Autor: |
Bjørge IM; Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal., de Sousa BM; Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, Aveiro 3810-193, Portugal., Patrício SG; Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal., Silva AS; Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal., Nogueira LP; Oral Research Laboratory, Institute of Clinical Dentistry, University of Oslo, Oslo 0455, Norway., Santos LF; Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal., Vieira SI; Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, Aveiro 3810-193, Portugal., Haugen HJ; Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo 0455, Norway., Correia CR; Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal., Mano JF; Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal. |
| Abstrakt: |
Fabrication of vascularized large-scale constructs for regenerative medicine remains elusive since most strategies rely solely on cell self-organization or overly control cell positioning, failing to address nutrient diffusion limitations. We propose a modular and hierarchical tissue-engineering strategy to produce bonelike tissues carrying signals to promote prevascularization. In these 3D systems, disc-shaped microcarriers featuring nanogrooved topographical cues guide cell behavior by harnessing mechanotransduction mechanisms. A sequential seeding strategy of adipose-derived stromal cells and endothelial cells is implemented within compartmentalized, liquefied-core macrocapsules in a self-organizing and dynamic system. Importantly, our system autonomously promotes osteogenesis and construct's mineralization while promoting a favorable environment for prevascular-like endothelial organization. Given its modular and self-organizing nature, our strategy may be applied for the fabrication of larger constructs with a highly controlled starting point to be used for local regeneration upon implantation or as drug-screening platforms. |
