Susceptibility of embryos of Biomphalaria tenagophila (Mollusca: Gastropoda) to infection by Pochonia chlamydosporia (Ascomycota: Sordariomycetes).

Autor: Castro LS; Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Av. P H Rolfs s/n, Viçosa, Minas Gerais, Brazil. lorenascast@gmail.com., Martins IVF; Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo-UFES, Alegre, ES, Brazil., Tunholi-Alves VM; Instituto de Veterinária (IV), Departamento de Parasitologia Animal (DPA), Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.; Universidade Estácio de Sá. Bolsista do Programa de Pesquisa e Produtividade da UNESA, Rio de Janeiro, RJ, Brasil., Amaral LS; Instituto de Veterinária (IV), Departamento de Parasitologia Animal (DPA), Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil., Pinheiro J; Instituto de Veterinária (IV), Departamento de Parasitologia Animal (DPA), Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil., de Araújo JV; Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Av. P H Rolfs s/n, Viçosa, Minas Gerais, Brazil., de Oliveira Monteiro CM; Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás-UFG, Goiânia, GO, Brazil., Tunholi VM; Departamento de Farmácia da Faculdade Multivix-Cachoeiro de Itapemirim, Rodovia Safra, bairro Monte Belo, Cachoeiro de Itapemirim, Espírito Santo, Brazil.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Apr 20; Vol. 204 (5), pp. 271. Date of Electronic Publication: 2022 Apr 20.
DOI: 10.1007/s00203-022-02894-x
Abstrakt: Schistosoma mansoni is a heteroxenous parasite, meaning that during its life cycle needs the participation of obligatory intermediate and definitive hosts. The larval development occurs in aquatic molluscs belonging to the Biomphalaria genus, leading to the formation of cercariae, which emerge to infect the final vertebrate host. For this reason, studies for control of the diseases caused by digenetic trematodes often focus on combating the snail hosts. Thus, the objective of this study was to evaluate the susceptibility of Biomphalaria tenagophila embryos to the fungus Pochonia chlamydosporia (isolate Pc-10). The entire experiment was conducted in duplicate, with five replicates for each repetition (five egg masses/replicate), utilizing a total of 100 egg masses, with 20-30 eggs/egg mass. At the end of 15 days, the egg masses were evaluated under a stereomicroscope to analyze the hatching of B. tenagophila embryos in both experimental groups. After days of interaction, the exposure to the fungal hyphae bodies significantly impaired the viability of the B. tenagophila eggs, inhibiting the embryogenesis process by 83.7% in relation to the control group. Transmission and scanning electron microscopic images revealed relevant structural alterations in the egg masses exposed to the hyphae action of the fungus, interfering in the development and hatching of the young snails under analysis. These results indicate the susceptibility of B. tenagophila embryos to the fungus P. chlamydosporia (isolate Pc-10) and suggest the potential of Pc-10 to be used in the control of intermediate host, for its ovicidal capacity and for being an ecologically viable option, but in vivo experiments become necessary.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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