High affinity zoledronate-based metal complex nanocrystals to potentially treat osteolytic metastases.

Autor: Quiñones Vélez G; Department of Chemistry, University of Puerto Rico Río Piedras San Juan Puerto Rico 00931 USA vilmali.lopez@upr.edu.; Crystallization Design Institute, Molecular Sciences Research Center, University of Puerto Rico San Juan Puerto Rico 00926 USA., Carmona-Sarabia L; Department of Chemistry, University of Puerto Rico Río Piedras San Juan Puerto Rico 00931 USA vilmali.lopez@upr.edu.; Crystallization Design Institute, Molecular Sciences Research Center, University of Puerto Rico San Juan Puerto Rico 00926 USA., Rivera Raíces AA; Crystallization Design Institute, Molecular Sciences Research Center, University of Puerto Rico San Juan Puerto Rico 00926 USA.; Department of Biology, University of Puerto Rico, Río Piedras San Juan Puerto Rico 00931 USA., Hu T; Department of Chemistry and the Molecular Design Institute, New York University 100 Washington Square East New York New York 10003-6688 USA., Peterson-Peguero EA; Department of Biology, University of Puerto Rico, Río Piedras San Juan Puerto Rico 00931 USA., López-Mejías V; Department of Chemistry, University of Puerto Rico Río Piedras San Juan Puerto Rico 00931 USA vilmali.lopez@upr.edu.; Crystallization Design Institute, Molecular Sciences Research Center, University of Puerto Rico San Juan Puerto Rico 00926 USA.
Jazyk: angličtina
Zdroj: Materials advances [Mater Adv] 2022 Feb 23; Vol. 3 (7), pp. 3251-3266. Date of Electronic Publication: 2022 Feb 23 (Print Publication: 2022).
DOI: 10.1039/d1ma01127h
Abstrakt: Formation of several materials, denoted as bisphosphonate-based coordination complexes (BPCCs), resulted from the reaction between clinically employed bisphosphonate, zoledronate (ZOLE) and bioactive metals (M 2+ = Ca 2+ , Mg 2+ and Zn 2+ ). Six ZOLE-based BPCCs were synthesized using different variables (M 2+  : ZOLE molar ratio, temperature, pH, and anion) and their structures were elucidated by single crystal X-ray diffraction (ZOLE-Ca forms I and II, ZOLE-Mg forms I and II, and ZOLE-Zn forms I and II). The dissolution of the ZOLE-based BPCCs was compared to that of ZOLE (Reclast®). Most of the ZOLE-based BPCCs (60-85%, in 18-24 h) present a lower dissolution and equilibrium solubility than ZOLE (∼100%, 30 min) in phosphate buffered saline (PBS), while a significantly higher dissolution is observed in acidic media (88% in 1 h). This suggests the ability to release the ZOLE content in a pH-dependent manner. Moreover, a phase inversion temperature (PIT)-nano-emulsion synthesis was performed, which demonstrated the ability to significantly decrease the crystal size of ZOLE-Ca form II from a micron-range (∼200 μm) to a nano-range (∼150 d nm), resulting in nano -Ca@ZOLE. Furthermore, low aggregation of nano -Ca@ZOLE in 10% fetal bovine serum (FBS) : PBS after 0, 24 and 48 h was demonstrated. Additionally, nano -Ca@ZOLE showed an ∼2.5 x more binding to hydroxyapatite (HA, 36%) than ZOLE (15%) in 1 d. The cytotoxicity of nano -Ca@ZOLE against MDA-MB-231 (cancer cell model) and hFOB 1.19 (normal osteoblast-like cell model) cell lines was investigated. The results demonstrated significant cell growth inhibition for nano -Ca@ZOLE against MDA-MB-231, specifically at a low concentration of 3.8 μM (%RCL = 55 ± 1%, 72 h). Under the same conditions, the nanocrystals did not present cytotoxicity against hFOB 1.19 (%RCL = 100 ± 2%). These results evidence that nano -ZOLE-based BPCCs possess viable properties in terms of structure, dissolution, stability, binding, and cytotoxicity, which render them suitable for osteolytic metastasis therapy.
Competing Interests: There are no conflicts of interest to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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