Chryseobacterium tagetis sp. nov., a plant growth promoting bacterium with an antimicrobial activity isolated from the roots of medicinal plant (Tagetes patula).

Autor: Chhetri G; Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea., Kim I; Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea., Kim J; Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea., So Y; Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea., Seo T; Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea. tseo@dongguk.edu.
Jazyk: angličtina
Zdroj: The Journal of antibiotics [J Antibiot (Tokyo)] 2022 Jun; Vol. 75 (6), pp. 312-320. Date of Electronic Publication: 2022 Apr 19.
DOI: 10.1038/s41429-022-00525-7
Abstrakt: A novel plant growth-promoting and indole acetic acid (IAA) producing strain designated RG1 T was isolated from the roots of Tagetes patula (marigold) collected from Goyang, South Korea. The cells of strain RG1 T is aerobic, yellow, Gram-stain-negative, pleomorphic and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RG1 T belongs to the genus Chryseobacterium and is closely related to Chryseobacterium gwangjuense THG-A18 T (98.6%). The strain produced IAA (70.5 µg ml -1 ) in the presence of L -tryptophan and showed antimicrobial activity against Gram-negative bacterium Xanthomonas campestris pv. campestris KACC 10377 T . The isolate had a significant positive effect on rice plant shoot and root growth. The novel strain RG1 T had a draft genome size of 4,430,189 bp, with ten scaffolds and 3969 protein-coding genes. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain RG1 T and other closely related members ranged from 21.5 to 36.6% and from 79.2 to 86.6%, respectively. The genomic DNA G + C content was 34.8 mol%. Furthermore, anti-SMASH analysis of the whole genome revealed six putative biosynthetic gene clusters responsible for various secondary metabolites. The major respiratory quinone was MK-6 and the major fatty acids were iso-C 15:0 , summed feature 3 (comprising C 16: 1 ω7c and/or C 16: 1 ω6c) and summed feature 9 (comprising iso-C 17: 1 ω9c and/or 10-methyl C 16:0 ). The major polar lipid is phosphatidylethanolamine. Based on the genotypic, chemotaxonomic and physiological data, strain RG1 T represents a novel species, for which the name Chryseobacterium tagetis sp. nov. is proposed. The type strain is designated as RG1 T ( = KCTC 82696 T  = NBRC 115057 T ).
(© 2022. The Author(s), under exclusive licence to the Japan Antibiotics Research Association.)
Databáze: MEDLINE
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