Sex-specific markers undetected in green sunfish Lepomis cyanellus using restriction-site associated DNA sequencing.

Autor: Teal CN; Arizona Cooperative Fish and Wildlife Research Unit, School of Natural Resources and the Environment, Tucson, Arizona, USA., Coykendall DK; Pacific States Marine Fisheries Commission, Eagle Fish Genetics Lab, Eagle, Idaho, USA.; Idaho Department of Fish and Game, Eagle Fish Genetics Lab, Eagle, Idaho, USA., Campbell MR; Idaho Department of Fish and Game, Eagle Fish Genetics Lab, Eagle, Idaho, USA., Eardley DL; Pacific States Marine Fisheries Commission, Eagle Fish Genetics Lab, Eagle, Idaho, USA.; Idaho Department of Fish and Game, Eagle Fish Genetics Lab, Eagle, Idaho, USA., Delomas TA; Pacific States Marine Fisheries Commission, Eagle Fish Genetics Lab, Eagle, Idaho, USA.; Idaho Department of Fish and Game, Eagle Fish Genetics Lab, Eagle, Idaho, USA., Shira JT; University of Arizona Genetics Core, Tucson, Arizona, USA., Schill DJ; Fisheries Management Solutions, Inc., Boise, Idaho, USA., Bonar SA; US Geological Survey, Arizona Cooperative Fish and Wildlife Research Unit, School of Natural Resources and the Environment, University of Arizona, ENR2, Tucson, Arizona, USA., Culver M; US Geological Survey, Arizona Cooperative Fish and Wildlife Research Unit, School of Natural Resources and the Environment, University of Arizona, ENR2, Tucson, Arizona, USA.
Jazyk: angličtina
Zdroj: Journal of fish biology [J Fish Biol] 2022 Jun; Vol. 100 (6), pp. 1528-1540. Date of Electronic Publication: 2022 May 06.
DOI: 10.1111/jfb.15063
Abstrakt: We used restriction-site associated DNA sequencing for SNP discovery and genotyping of known-sex green sunfish Lepomis cyanellus DNA samples to search for sex-diagnostic single nucleotide polymorphisms (SNPs) and restriction-site associated sequences present in one sex and absent in the other. The bioinformatic analyses discovered candidate SNPs and sex-specific restriction-site associated sequences that fit patterns of male or female heterogametic sex determination systems. However, when primers were developed and tested, no candidates reliably identified phenotypic sex. The top performing SNP candidate (ZW_218) correlated with phenotypic sex 63.0% of the time and the presence-absence loci universally amplified in both sexes. We recommend further investigations that interrogate a larger fraction of the L. cyanellus genome. Additionally, studies on the effect of temperature and rearing density on sex determination, as well as breeding of sex-reversed individuals, could provide more insights into the sex determination system of L. cyanellus.
(© 2022 Fisheries Society of the British Isles.)
Databáze: MEDLINE
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