Evaluation of mutagenesis, necrosis and apoptosis induced by omeprazole in stomach cells of patients with gastritis.

Autor: da Mata AMOF; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, PI, 64.049-550, Brazil., Paz MFCJ; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, PI, 64.049-550, Brazil., de Menezes APM; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, PI, 64.049-550, Brazil., Dos Reis AC; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, PI, 64.049-550, Brazil., da Silva Souza B; Federal University of Delta Do Parnaíba, Parnaiba, PI, 64202-020, Brazil., de Carvalho Sousa CD; Getúlio Vargas Hospital, Teresina, PI, 64001-020, Brazil., Machado SA; Getúlio Vargas Hospital, Teresina, PI, 64001-020, Brazil., Medeiros TSG; Getúlio Vargas Hospital, Teresina, PI, 64001-020, Brazil., Sarkar C; Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University Bangladesh, Gopalganj, 8100, Bangladesh., Islam MT; Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University Bangladesh, Gopalganj, 8100, Bangladesh. dmt.islam@bsmrstu.edu.bd., Sharifi-Rad J; Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. javad.sharifirad@gmail.com.; Facultad de Medicina, Universidad del Azuay, Cuenca, Ecuador. javad.sharifirad@gmail.com., Daştan SD; Department of Biology, Faculty of Science, Sivas Cumhuriyet University, 58140, Sivas, Turkey.; Beekeeping Development Application and Research Center, Sivas Cumhuriyet University, 58140, Sivas, Turkey., Alshehri MM; Pharmaceutical Care Department, Ministry of National Guard-Health Affairs, Riyadh, Saudi Arabia., de Castro E Sousa JM; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, PI, 64.049-550, Brazil., de Carvalho Melo Cavalcante AA; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, PI, 64.049-550, Brazil.
Jazyk: angličtina
Zdroj: Cancer cell international [Cancer Cell Int] 2022 Apr 18; Vol. 22 (1), pp. 154. Date of Electronic Publication: 2022 Apr 18.
DOI: 10.1186/s12935-022-02563-5
Abstrakt: Background: Gastritis is a superficial and prevalent inflammatory lesion that is considered a public health concern once can cause gastric ulcers and gastric cancer, especially when associated with Helicobacter pylori infection. Proton pump inhibitors, such as omeprazole, are the most widely used drugs to treat this illness. The aim of the study was evaluate cytogenetic effects of omeprazole in stomach epithelial cells of patients with gastritis in presence and absence of H. pylori, through cytogenetic biomarkers and catalse and superoxide dismutase analysis.
Methods: The study included 152 patients from the Gastroenterology Outpatient Clinic of Hospital Getúlio Vargas, Teresina-Brazil, that reported continuous and prolonged omeprazole use in doses of 20, 30 and 40 mg/kg. The participants were divided into groups: (1) patients without gastritis (n = 32); (2) patients without gastritis but with OME use (n = 24); (3) patients with gastritis (n = 26); (4) patients with gastritis undergoing OME therapy (n = 26); (5) patients with gastritis and H. pylori (n = 22) and (6) patients with gastritis and H. pylori on OME therapy (n = 22).
Results: OME induced cytogenetic imbalance in the stomach epithelium through the formation of micronuclei (group 6 > 1, 2, 3, 4, 5; group 5 > 1, 2, 3; group 4 > 1, 2, 3); bridges (groups 4 and 6 > 1, 2, 3, 5 and group 2 > 3, 5); buds (groups 2,4,6 > , 1, 3, 5); binucleated cells (group 6 > 1, 2, 3, 4, 5; group 4 > 1, 2, 3); (groups 2 and 3 > 1); picnoses (group 6 > 1, 2, 3, 4, 5), groups 2 and 5 > 1, 3; group 4 > 1, 2, 3, 5); cariorrexis (groups 6 and 4 > 1, 2, 3, 5; groups 2, 3, 5 > 1) and karyolysis (groups 2, 4, and 6 > 1, 3, 5; groups 3 and 5 > 1). The OME cytogenetic instability was associated with H. pylori infection, indicating clastogenic/aneugenic effects, chromosomes alterations, gene expression changes, cytotoxicity and apoptosis.
Conclusions: The cytogenetic changescan be attributed to several mechanisms that are still unclear, including oxidative damage, as observed by increased catalase and superoxide dismutase expresion. Positive correlations between antioxidant enzymes were found with micronuclei formation, and were negative for picnoses. Thus, the continuous and prolonged omeprazole use induces genetic instability, which can be monitored through cytogenetic analyzes, as precursor for gastric cancer.
(© 2022. The Author(s).)
Databáze: MEDLINE
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