Microarray analysis and PCR validation of genes associated with facultative parthenogenesis in Meleagris gallopavo (Turkey).

Autor: Bhanja SK; ICAR-Central Avian Research Institute, Izatnagar, 243 122, India. Electronic address: subratcari@gmail.com., Goel A; ICAR-Central Avian Research Institute, Izatnagar, 243 122, India. Electronic address: genesakshat@gmail.com., Mehra M; ICAR-Central Avian Research Institute, Izatnagar, 243 122, India., Bag S; ICAR-Indian Veterinary Research Institute, Izatnagar, 243 122, India., Kharche SD; ICAR-Central Institute for Research on Goats, Makhdoom, Farah, 281 122, India., Malakar D; Animal Biotechnology Center, ICAR-National Dairy Research Institute, Karnal, India., Dash B; ICAR-Indian Veterinary Research Institute, Izatnagar, 243 122, India.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2022 Jul 01; Vol. 186, pp. 86-94. Date of Electronic Publication: 2022 Mar 29.
DOI: 10.1016/j.theriogenology.2022.03.027
Abstrakt: A cDNA microarray containing 43,661 differentially expressed genes was carried out on the blastoderm of fertilized and facultative parthenogenic turkey embryos at different hours of development. The total number of up-regulated (UR) and down-regulated (DR) genes at 0, 12, and 24 h of development were 725 and 1436, 942 and 599, and 589 and 1044, respectively. Common genes between 0 and 12 h, 12 and 24 h, and 0 and 24 h were 55, 67, and 110, respectively. The proportion of genes showing above 50-fold UR and DR at 0, 12, and 24 h of development were 2.0% and 1.5%, 0.5% and 1.2%, and 0.2% and 1.1%, respectively. Eight UR genes were validated (APOA1, THRAP3, ARL14EP, PSAP, MOG, MYBPC2, MTIF3 and EDG4) and relative expression of six of them was significantly higher (P ≤ 0.05) in parthenogenic embryos, while two genes showed non-significant (P ≥ 0.05) variation. The expression of BCL11A, PRP4B, TCP1, and TPI1 genes was significantly (P ≤ 0.05) DR in parthenotes in the micro-array study, while the TCP1 gene was up-regulated, and there was no variation in TPI1 gene expression in the PCR validation study. In conclusion, our findings demonstrate differential expression of a large number of genes in parthenotes at different stages of embryo development compared to fertilized embryos. Up-regulation of APOA1, MYBPC2, TCP1, and THRAP3 genes, suggest their crucial role in spontaneous facultative parthenogenic development in turkey birds.
Competing Interests: Declaration of competing interest The authors declare that no conflict of interest could be perceived as prejudicing the impartiality of the research report.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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