Fast Ion-Beam Inactivation of Viruses, Where Radiation Track Structure Meets RNA Structural Biology.
| Autor: | Villagomez-Bernabe B; The Dalton Cumbrian Facility and the Department of Chemistry, The University of Manchester, Westlakes Science & Technology Park, Moor Row, Cumbria, CA24 3HA, United Kingdom., Chan SW; School of Biological Sciences, Faculty of Biology, Medicine & Health, The University of Manchester, Manchester Academic Health Science Centre, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PL, United Kingdom., Coulter JA; School of Pharmacy, Queen's University Belfast, Belfast, BT9 7BL., Roseman AM; School of Biological Sciences, Faculty of Biology, Medicine & Health, The University of Manchester, Manchester Academic Health Science Centre, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PL, United Kingdom., Currell FJ; The Dalton Cumbrian Facility and the Department of Chemistry, The University of Manchester, Westlakes Science & Technology Park, Moor Row, Cumbria, CA24 3HA, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | Radiation research [Radiat Res] 2022 Jul 01; Vol. 198 (1), pp. 68-80. |
| DOI: | 10.1667/RADE-21-00133.1 |
| Abstrakt: | Here we show an interplay between the structures present in ionization tracks and nucleocapsid RNA structural biology, using fast ion-beam inactivation of the severe acute respiratory syndrome coronavirus (SARS-CoV) virion as an example. This interplay could be a key factor in predicting dose-inactivation curves for high-energy ion-beam inactivation of virions. We also investigate the adaptation of well-established cross-section data derived from radiation interactions with water to the interactions involving the components of a virion, going beyond the density-scaling approximation developed previously. We conclude that solving one of the grand challenges of structural biology - the determination of RNA tertiary/quaternary structure - is linked to predicting ion-beam inactivation of viruses and that the two problems can be mutually informative. Indeed, our simulations show that fast ion beams have a key role to play in elucidating RNA tertiary/quaternary structure. (©2022 by Radiation Research Society. All rights of reproduction in any form reserved.) |
| Databáze: | MEDLINE |
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