SARS-CoV-2 RNA is enriched by orders of magnitude in primary settled solids relative to liquid wastewater at publicly owned treatment works.

Autor:	Kim S; Dept of Civil and Environmental Engineering, Stanford University Stanford CA 94305 USA aboehm@stanford.edu., Kennedy LC; Dept of Civil and Environmental Engineering, Stanford University Stanford CA 94305 USA aboehm@stanford.edu., Wolfe MK; Dept of Civil and Environmental Engineering, Stanford University Stanford CA 94305 USA aboehm@stanford.edu.; Rollins School of Public Health, Emory University Atlanta GA 30329 USA., Criddle CS; Dept of Civil and Environmental Engineering, Stanford University Stanford CA 94305 USA aboehm@stanford.edu., Duong DH; Verily Life Sciences South San Francisco CA 94080 USA., Topol A; Verily Life Sciences South San Francisco CA 94080 USA., White BJ; Verily Life Sciences South San Francisco CA 94080 USA., Kantor RS; Dept of Civil and Environmental Engineering, University of California Berkeley CA 94720 USA., Nelson KL; Dept of Civil and Environmental Engineering, University of California Berkeley CA 94720 USA., Steele JA; Southern California Coastal Water Research Project Costa Mesa CA 92626 USA., Langlois K; Southern California Coastal Water Research Project Costa Mesa CA 92626 USA., Griffith JF; Southern California Coastal Water Research Project Costa Mesa CA 92626 USA., Zimmer-Faust AG; Southern California Coastal Water Research Project Costa Mesa CA 92626 USA., McLellan SL; School of Freshwater Sciences, University of Wisconsin-Milwaukee Milwaukee WI 53204 USA., Schussman MK; School of Freshwater Sciences, University of Wisconsin-Milwaukee Milwaukee WI 53204 USA., Ammerman M; Department of Civil and Environmental Engineering, University of Michigan Ann Arbor MI 48109 USA., Wigginton KR; Department of Civil and Environmental Engineering, University of Michigan Ann Arbor MI 48109 USA., Bakker KM; Department of Epidemiology, University of Michigan Ann Arbor MI 48109 USA., Boehm AB; Dept of Civil and Environmental Engineering, Stanford University Stanford CA 94305 USA aboehm@stanford.edu.
Jazyk:	angličtina
Zdroj:	Environmental science : water research & technology [Environ Sci (Camb)] 2022 Feb 15; Vol. 8 (4), pp. 757-770. Date of Electronic Publication: 2022 Feb 15 (Print Publication: 2022).
DOI:	10.1039/d1ew00826a
Abstrakt:	Wastewater-based epidemiology has gained attention throughout the world for detection of SARS-CoV-2 RNA in wastewater to supplement clinical testing. Raw wastewater consists of small particles, or solids, suspended in liquid. Methods have been developed to measure SARS-CoV-2 RNA in the liquid and the solid fraction of wastewater, with some studies reporting higher concentrations in the solid fraction. To investigate this relationship further, six laboratories collaborated to conduct a study across five publicly owned treatment works (POTWs) where both primary settled solids obtained from primary clarifiers and raw wastewater influent samples were collected and quantified for SARS-CoV-2 RNA. Settled solids and influent samples were processed by participating laboratories using their respective methods and retrospectively paired based on date of collection. SARS-CoV-2 RNA concentrations, on a mass equivalent basis, were higher in settled solids than in influent by approximately three orders of magnitude. Concentrations in matched settled solids and influent were positively and significantly correlated at all five POTWs. RNA concentrations in both settled solids and influent were correlated to COVID-19 incidence rates in the sewersheds and thus representative of disease occurrence; the settled solids methods appeared to produce a comparable relationship between SARS-CoV-2 RNA concentration measurements and incidence rates across all POTWs. Settled solids and influent methods showed comparable sensitivity, N gene detection frequency, and calculated empirical incidence rate lower limits. Analysis of settled solids for SARS-CoV-2 RNA has the advantage of using less sample volume to achieve similar sensitivity to influent methods. Competing Interests: Bradley J. White, Aaron Topol, and Dorothea H. Duong are employees of Verily Life Sciences. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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