Characterisation of the Introgression of Brassica villosa Genome Into Broccoli to Enhance Methionine-Derived Glucosinolates and Associated Health Benefits.

Autor:	Neequaye M; Quadram Institute Bioscience, Norwich, United Kingdom.; John Innes Centre, Norwich, United Kingdom., Steuernagel B; John Innes Centre, Norwich, United Kingdom., Saha S; Quadram Institute Bioscience, Norwich, United Kingdom., Trick M; John Innes Centre, Norwich, United Kingdom., Troncoso-Rey P; Quadram Institute Bioscience, Norwich, United Kingdom., van den Bosch F; Bayer, Wageningen, Netherlands., Traka MH; Quadram Institute Bioscience, Norwich, United Kingdom., Østergaard L; John Innes Centre, Norwich, United Kingdom., Mithen R; Quadram Institute Bioscience, Norwich, United Kingdom.; Liggins Institute, University of Auckland, Auckland, New Zealand.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Apr 01; Vol. 13, pp. 855707. Date of Electronic Publication: 2022 Apr 01 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.855707
Abstrakt:	Broccoli cultivars that have enhanced accumulation of methionine-derived glucosinolates have been developed through the introgression of a novel allele of the MYB28 transcription factor from the wild species Brassica villosa . Through a novel k-mer approach, we characterised the extent of the introgression of unique B. villosa genome sequences into high glucosinolate broccoli genotypes. RNAseq analyses indicated that the introgression of the B. villosa MYB28 C2 allele resulted in the enhanced expression of the MYB28 transcription factor, and modified expression of genes associated with sulphate absorption and reduction, and methionine and glucosinolate biosynthesis when compared to standard broccoli. A adenine-thymine (AT) short tandem repeat (STR) was identified within the 5' untranslated region (UTR) B. villosa MYB28 allele that was absent from two divergent cultivated forms of Brassica oleracea , which may underpin the enhanced expression of B. villosa MYB28 . Competing Interests: The broccoli with elevated glucoraphanin is the subject of patents filed by Plant Bioscience Limited (PBL), the technology transfer company of the John Innes Centre. RM and MHT are inventors named on these patents. FB was employed by Bayer. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Neequaye, Steuernagel, Saha, Trick, Troncoso-Rey, van den Bosch, Traka, Østergaard and Mithen.)
Databáze:	MEDLINE
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