Molecular identification and lipid mobilization role of adipokinetic hormone receptor in Spodoptera litura (F.).

Autor:	Dong X; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China.; Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China., Chen J; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China., Xu R; Tobacco Research Institute of Hubei Province, Wuhan 430002, Hubei, China., Li X; Tobacco Research Institute of Hubei Province, Wuhan 430002, Hubei, China., Wang Y; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China., Pan X; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China., Zhang C; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China., Li Y; Tobacco Research Institute of Hubei Province, Wuhan 430002, Hubei, China., Wang F; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China., Li C; Hubei Engineering Research Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China.
Jazyk:	angličtina
Zdroj:	Bulletin of entomological research [Bull Entomol Res] 2022 Dec; Vol. 112 (6), pp. 758-765. Date of Electronic Publication: 2022 Apr 18.
DOI:	10.1017/S0007485322000141
Abstrakt:	Energy homeostasis is essential for organisms to maintain fluctuation in energy accumulation, mobilization. Lipids as the main energy reserve in insects, their metabolism is under the control of many physiological program. This study aimed to determine whether the adipokinetic hormone receptor (AKHR) was involved in the lipid mobilization in the Spodoptera litura . A full-length cDNA encoding AKHR was isolated from S. litura . The Sl AKHR protein has a conserved seven-transmembrane domain which is the character of a putative G protein receptor. Expression profile investigation revealed that SlAKHR mRNA was highly expressed in immatural stage and abundant in fat body in newly emerged female adults. Knockdown of SlAKHR expression was achieved through RNAi by injecting double-stranded RNA (dsRNA) into the 6th instar larvae. The content of triacylgycerol (TAG) in the fat body increased significantly after the SlAKHR gene was knockdown. And decrease of TAG releasing to hemolymph with increase of free fatty acid (FFA) in hemolymph were observed when the SlAKHR gene was knowned-down. In addition, lipid droplets increased in fat body was also found. These results suggested that SlAKHR is critical for insects to regulate lipids metabolism.
Databáze:	MEDLINE
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