FRET-mediated quenching of BODIPY fluorescent nanoparticles by methylene blue and its application to bacterial imaging.

Autor: Si Y; PPSM, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France.; LBPA, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France.; Epigenetic Chemical Biology, CNRS UMR3523, Institut Pasteur, 28 Rue du Dr Roux, 75015, Paris, France., Grazon C; PPSM, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France.; University of Bordeaux, CNRS, Bordeaux INP, ISM, UMR 5255, 33400, Talence, France., Clavier G; PPSM, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France., Audibert JF; PPSM, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France., Sclavi B; LBPA, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France. bianca.sclavi@sorbonne-universite.fr.; LCQB, CNRS UMR 7238, Sorbonne Université, 4 Place Jussieu, 75005, Paris, France. bianca.sclavi@sorbonne-universite.fr., Méallet-Renault R; PPSM, Université Paris-Saclay, ENS Paris-Saclay, CNRS, 91190, Gif-sur-Yvette, France. rachel.meallet-renault@universite-paris-saclay.fr.; ISMO, Université Paris-Saclay, CNRS, 91405, Orsay, France. rachel.meallet-renault@universite-paris-saclay.fr.
Jazyk: angličtina
Zdroj: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2022 Jul; Vol. 21 (7), pp. 1249-1255. Date of Electronic Publication: 2022 Apr 15.
DOI: 10.1007/s43630-022-00215-1
Abstrakt: High resolution and a good signal to noise ratio are a requirement in cell imaging. However, after labelling with fluorescent entities, and after several washing steps, there is often an unwanted fluorescent background that reduces the images resolution. For this purpose, we developed an approach to remove the signal from extra-cellular fluorescent nanoparticles (FNPs) during bacteria imaging, without the need for any washing steps. Our idea is to use methylene blue to quench > 90% of the emission of BODIPY-based fluorescent polymer nanoparticle by a FRET process. This "Hide-and-Seek Game" approach offers a novel strategy to apply fluorescence quenching in bioimaging to improve image accuracy.
(© 2022. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.)
Databáze: MEDLINE
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