An alginate-based encapsulation system for delivery of therapeutic cells to the CNS.

Autor:	Eleftheriadou D; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Evans RE; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Atkinson E; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Abdalla A; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Gavins FKH; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Boyd AS; UCL Institute of Immunity and Transplantation, Royal Free Hospital London UK., Williams GR; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Knowles JC; Biomaterials & Tissue Engineering, UCL Eastman Dental Institute London UK., Roberton VH; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk., Phillips JB; UCL Centre for Nerve Engineering, University College London London UK.; UCL School of Pharmacy, University College London London WC1N 1AX UK jb.phillips@ucl.ac.uk.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2022 Feb 01; Vol. 12 (7), pp. 4005-4015. Date of Electronic Publication: 2022 Feb 01 (Print Publication: 2022).
DOI:	10.1039/d1ra08563h
Abstrakt:	Treatment options for neurodegenerative conditions such as Parkinson's disease have included the delivery of cells which release dopamine or neurotrophic factors to the brain. Here, we report the development of a novel approach for protecting cells after implantation into the central nervous system (CNS), by developing dual-layer alginate beads that encapsulate therapeutic cells and release an immunomodulatory compound in a sustained manner. An optimal alginate formulation was selected with a view to providing a sustained physical barrier between engrafted cells and host tissue, enabling exchange of small molecules while blocking components of the host immune response. In addition, a potent immunosuppressant, FK506, was incorporated into the outer layer of alginate beads using electrosprayed poly-ε-caprolactone core-shell nanoparticles with prolonged release profiles. The stiffness, porosity, stability and ability of the alginate beads to support and protect encapsulated SH-SY5Y cells was demonstrated, and the release profile of FK506 and its effect on T-cell proliferation in vitro was characterized. Collectively, our results indicate this multi-layer encapsulation technology has the potential to be suitable for use in CNS cell delivery, to protect implanted cells from host immune responses whilst providing permeability to nutrients and released therapeutic molecules. Competing Interests: None of the authors have any relevant conflicts of interest to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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