Hypoglycemic effect and toxicity of the dry extract of Eugenia biflora (L.) DC. leaves.

Autor: Oliveira ESC; Núcleo de Estudos Químicos de Micromoléculas da Amazônia - NEQUIMA, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil., Acho LDR; Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas Manaus, Amazonas, Brazil., da Silva BJP; Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas Manaus, Amazonas, Brazil., Morales-Gamba RD; Programa de Pós-graduação em Zoologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil., Pontes FLD; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., do Rosário AS; Engenharia Florestal, Campus Paragominas, Universidade Federal Rural da Amazônia, Paragominas, Pará, Brazil., Bezerra JA; Núcleo de Estudos Químicos de Micromoléculas da Amazônia - NEQUIMA, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil; Departamento de Química, Ambiente e Alimentos, Instituto Federal de Educação, Ciência e Tecnologia do Amazonas, Manaus, Amazonas, Brazil., Campos FR; Núcleo de Estudos Químicos de Micromoléculas da Amazônia - NEQUIMA, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil; Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., Barcellos JFM; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil., Lima ES; Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas Manaus, Amazonas, Brazil., Machado MB; Núcleo de Estudos Químicos de Micromoléculas da Amazônia - NEQUIMA, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil. Electronic address: marcosmachado@ufam.edu.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2022 Jul 15; Vol. 293, pp. 115276. Date of Electronic Publication: 2022 Apr 11.
DOI: 10.1016/j.jep.2022.115276
Abstrakt: Ethnopharmacological Relevance: The leaves of Eugenia biflora (Myrtaceae) are traditionally used by Amazonian populations for the control of diabetes. However, their chemical composition has not yet been described and pharmacological evidence has not been reported.
Objective: This study aimed to identify the chemical constituents and evaluate the hypoglycemic and toxic effect of the dry extract of the E. biflora leaves (DEEB).
Materials and Methods: DEEB, obtained by infusion, was analyzed using LC-HRMS and NMR, whose the catechin flavonoid was quantified using NMR. The antidiabetic effect of DEEB was evaluated according to its inhibition of the enzymes α-amylase and α-glucosidase, as well as the content of total phenols, free radical scavengingand antiglycation activities, and its in vitro cell viability. Oral maltose tolerance and chronic multiple dose tests (28 days) in streptozotocin-induced diabetic mice (STZ) were performed. The hypoglycemic effect and toxicity of this extract were evaluated in the multiple dose assay. Biochemical parameters, hemolysis, and levels of the thiobarbituric acid reactive species in the liver were investigated and histopathological analyses of the kidneys and liver were performed.
Results: Eight phenolic compounds were identified, with catechin (15.5 ± 1.7 mg g-1) being the majority compound and a possible chemical marker of DEEB. The extract showed inhibition activity of the enzyme α-glucosidase. Chronic administration of DEEB (50 mg/kg of body weight) reduced glucose levels in diabetic animals, similar to acarbose; however, DEEB (100 and 200 mg/kg bw) caused premature death of mice by D22 of the treatment. Our data indicate that one of the mechanisms of toxicity in DEEB may be related to the aggravation of oxidative stress in the liver. This histopathological study indicated that DEEB failed to minimize the progression of the toxicity of diabetes caused by STZ.
Conclusions: This study demonstrated the hypoglycemic potential of E. biflora leaves. However, the prolonged use of this tea can be harmful to its users due to its considerable toxicity, which needs to be better investigated.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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