Microbial diversity and composition in acidic sediments of freshwater finfish culture ponds fed with two types of feed: a metagenomic approach.

Autor: Kusunur AB; ICAR-Central Institute of Fisheries Technology, Visakhapatnam Research Centre, Visakhapatnam, India., Velayudhan LK; Former Principal Scientist, ICAR-Central Institute of Fisheries Technology, Cochin, India., Vaiyapuri M; ICAR-Central Institute of Fisheries Technology, Cochin, India., Gaurav R; ICAR-National Bureau of Fish Genetic Resources, Lucknow, India., Tripathi G; ICAR-Central Institute of Fisheries Education, Mumbai, India., Kurcheti PP; ICAR-Central Institute of Fisheries Education, Mumbai, India., Badireddy MR; ICAR-Central Institute of Fisheries Technology, Visakhapatnam Research Centre, Visakhapatnam, India., Joseph TC; ICAR-Central Institute of Fisheries Technology, Cochin, India.
Jazyk: angličtina
Zdroj: Letters in applied microbiology [Lett Appl Microbiol] 2022 Jul; Vol. 75 (1), pp. 171-181. Date of Electronic Publication: 2022 Apr 20.
DOI: 10.1111/lam.13720
Abstrakt: Microbial community profile associated with acidic pond sediments (APS) (pH = 3·0-4·5) of freshwater finfish aquaculture ponds (n = 8) was investigated. Sediment DNA extracted from the eight APS were subjected to high-throughput sequencing of V3 and V4 regions which yielded 7236 operational taxonomic units (OTUs) at a similarity of 97%. Overall results showed higher proportion of bacterial OTUs than archaeal OTUs in all the APS. Euryarchaeota (23%), Proteobacteria (19%), Chloroflexi (17%), Crenarchaeota (5·3%), Bacteroidetes (4·8%), Nitrospirae (3·2%), Nanoarchaeaeota (3%) which together constituted 75% of the microbial diversity. At the genus level, there was high preponderance of methanogens namely Methanolinea (5·4%), Methanosaeta (4·5%) and methanotrops, Bathyarchaeota (5%) in APS. Moreover, the abundant phyla in the APS were not drastically affected by the administration of chicken slaughter waste (R-group ponds) and commercial fish feed (C-group ponds), since 67% of the OTUs generated remained common in the APS of both the groups of ponds. There was a minimal difference of 24-26% of OTUs between C-group and R-group ponds, suggesting the existence of a core microbial community in these ponds driven by acidic pH over the years. This study concludes that microbial diversity in pond sediment was influenced to a lesser extent by the addition of chicken slaughter waste but was majorly driven by acidic nature of the pond.
(© 2022 The Society for Applied Microbiology.)
Databáze: MEDLINE
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