UHPLC-MS/MS Studies and Antiproliferative Effects in Breast Cancer Cells of Mexican Sargassum.

Autor:	Fragoso-Vázquez MJ; Departamento de Quimica Organica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, Col. Casco de Santo Tomas, Mexico City, CP 11340, Mexico., Duclosel D; Laboratorio de Biofísica y Biocatálisis, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Casco de Santo Tomás, 11340, Mexico City, Mexico., Rosales-Hernández MC; Laboratorio de Biofísica y Biocatálisis, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Casco de Santo Tomás, 11340, Mexico City, Mexico., Estrada-Pérez A; Laboratorio de Diseno y Desarrollo de Nuevos Farmacos e Innovacion Biotecnológica de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico. Plan de San Luis Y Diaz Mirón S/N, Col. Casco de Santo Tomás, México City, CP 11340, Mexico., Mendoza-Figueroa HL; Laboratorio de Diseno y Desarrollo de Nuevos Farmacos e Innovacion Biotecnológica de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico. Plan de San Luis Y Diaz Mirón S/N, Col. Casco de Santo Tomás, México City, CP 11340, Mexico., Olivares-Corichi I; Laboratory of Oxidative Stress in Research and Graduate Studies Section, Instituto Politécnico Nacional, Escuela Superior de Medicina, Mexico City, México., Mendieta-Wejebe JE; Laboratorio de Biofísica y Biocatálisis, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Casco de Santo Tomás, 11340, Mexico City, Mexico., Reyes-López CA; Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Laboratorio de Bioquímica Estructural. Ciudad de México, México., Velasco-Quijano JS; Laboratorio de Diseno y Desarrollo de Nuevos Farmacos e Innovacion Biotecnológica de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico. Plan de San Luis Y Diaz Mirón S/N, Col. Casco de Santo Tomás, México City, CP 11340, Mexico., Gil-Ruiz LA; Laboratorio de Diseno y Desarrollo de Nuevos Farmacos e Innovacion Biotecnológica de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico. Plan de San Luis Y Diaz Mirón S/N, Col. Casco de Santo Tomás, México City, CP 11340, Mexico., Correa-Basurto J; Laboratorio de Diseno y Desarrollo de Nuevos Farmacos e Innovacion Biotecnologica de la Escuela Superior de Medicina, Instituto Politecnico Nacional, Mexico. Plan de San Luis Y Diaz Miron S/N, Col. Casco de Santo Tomas, Mexico City, CP 11340, Mexico.
Jazyk:	angličtina
Zdroj:	Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2023; Vol. 23 (1), pp. 76-86.
DOI:	10.2174/1871520622666220412125740
Abstrakt:	Background: Sargassum is a marine organism that, under specific conditions, drastically increases its population damaging the environment and risking other organisms. However, sargassum could represent a source of bioactive compounds to treat different diseases such as cancer. Thus, aqueous, ethanolic, and ethyl acetate extracts of sargassum from Playa del Carmen, Mexico, were subjected to metabolomic and antiproliferative assays in breast cancer cells. Objective: To evaluate the biological effect of different extracts of sargassum, its toxicity over Artemia salina and its antiproliferative effect tested in MCF-7, MDA-MB-231, and NIH3T3 cell lines. Finally, using UHPLC-MS/MS to identify the metabolites in each extract to correlate them with its antiproliferative effect. Methods: The sargassum sample collection was carried out in September at three different points in Playa del Carmen, Quintana Roo, Mexico. The aqueous, ethanolic, and ethyl acetate extracts of Mexican sargassum were obtained by evaporation of solvent and lyophilization. Then, these extracts were evaluated in the cytotoxicity bioassay of Artemia salina. Next, its antiproliferative effect was assessed in MCF-7, MDA-MB-231, and NIH3T3 cell lines. Using UHPLC-MS/MS, the metabolites present in each extract were identified. Finally, docking studies on sphingosine kinase 1 (PDB ID: 3VZB) of sphingosine were carried out. Results: The extracts from sargassum showed a greater effect in the antiproliferative assays in cells than in cytotoxic assays in Artemia salina. The ethanolic extract obtained from sargassum showed the best antiproliferative activity in MCF7 and MDA-MB-231 cells. Despite its antiproliferative effect on NIH3T3 cells, an additional extract is required indicating that this extract has compounds that could have a better effect on cancer cells in fibroblast (NIH3T3). The UHPLC-MS/MS of ethanolic and the ethyl acetate extract showed that these extracts have compounds such as sphinganine C16, N, N-Dimethylsphingosine compound, and that it could be possible that the effect observed is due to their metabolites which could be ligands for the sphingosine kinase 1 as demonstrated by docking studies. Conclusion: The ethanolic extract obtained from sargassum has better antiproliferative activity, despite not having a cytotoxic effect in Artemia salina. The antiproliferative effect could be related to the sphinganine C16, N,NDimethylphingosine identified with more abundance by UHPLC-MS/MS. In addition, these metabolites could be targets of sphingosine kinase 1. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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