Molecular and cytological profiling of biological aging of mouse cochlear inner and outer hair cells.

Autor:	Liu H; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA., Giffen KP; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA; Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University/University of Georgia Medical Partnership, Athens, GA, USA. Electronic address: kgiffen@augusta.edu., Chen L; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA; Chongqing Academy of Animal Sciences, Chongqing 402460, China., Henderson HJ; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA., Cao TA; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA., Kozeny GA; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA., Beisel KW; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA., Li Y; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA; Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China. Electronic address: alinaliyi@163.com., He DZ; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA. Electronic address: hed@creighton.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2022 Apr 12; Vol. 39 (2), pp. 110665.
DOI:	10.1016/j.celrep.2022.110665
Abstrakt:	Age-related hearing loss (ARHL) negatively impacts quality of life in the elderly population. The prevalent cause of ARHL is loss of mechanosensitive cochlear hair cells (HCs). The molecular and cellular mechanisms of HC degeneration remain poorly understood. Using RNA-seq transcriptomic analyses of inner and outer HCs isolated from young and aged mice, we show that HC aging is associated with changes in key molecular processes, including transcription, DNA damage, autophagy, and oxidative stress, as well as genes related to HC specialization. At the cellular level, HC aging is characterized by loss of stereocilia, shrinkage of HC soma, and reduction in outer HC mechanical properties, suggesting that functional decline in mechanotransduction and cochlear amplification precedes HC loss and contributes to ARHL. Our study reveals molecular and cytological profiles of aging HCs and identifies genes such as Sod1, Sirt6, Jund, and Cbx3 as biomarkers and potential therapeutic targets for ameliorating ARHL. Competing Interests: Declaration of interests The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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