Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching.

Autor:	Silva IJP; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Departamento de Endodontia, Araçatuba, SP, Brasil., Cintra LTA; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Departamento de Endodontia, Araçatuba, SP, Brasil., Ervolino E; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Departamento de Ciências Básicas, Araçatuba, SP, Brasil., Chaves HGDS; Universidade Federal de Minas Gerais (UFMG), Faculdade de Odontologia, Departamento de Odontologia Restauradora, Belo Horizonte, MG, Brasil., Sivieri-AraúJo G; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Departamento de Endodontia, Araçatuba, SP, Brasil., Briso ALF; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Departamento de Odontologia Restauradora, Araçatuba, SP, Brasil., Cosme-Silva L; Universidade Federal de Alagoas (UFAL), Faculdade de Odontologia, Maceió, AL, Brasil., Benetti F; Universidade Federal de Minas Gerais (UFMG), Faculdade de Odontologia, Departamento de Odontologia Restauradora, Belo Horizonte, MG, Brasil.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2022 Apr 08; Vol. 30, pp. e20210559. Date of Electronic Publication: 2022 Apr 08 (Print Publication: 2022).
DOI:	10.1590/1678-7757-2021-0559
Abstrakt:	Objectives: To evaluate the influence of photobiomodulation with infrared laser (IRL) in the rat pulp tissue after bleaching, considering the immunolabeling of interleukin (IL)-23 and hypoxia-inducible factor (HIF)-1α. Methodology: The right and left molars of forty rats were divided into groups: Control - with placebo gel and Bleached - with 35% hydrogen peroxide (H2O2). Half of the rats received one IRL application on both sides, establishing a split-mouth design, which resulted in 4 groups with 20 hemi-maxillae each: Control, Bleach, IRL, and Bleached-IRL. Rats (n=10) from each group were euthanized, at 2- and 30-days mark, and the pulp tissue was evaluated using inflammation and immunolabeling scores. Wilcoxon and Mann-Whitney statistical tests were performed (p<0.05). Results: At the 2-days mark, the Bleached group had severe inflammation and necrosis in the occlusal thirds of the pulp, and moderate to severe inflammation in cervical third, whereas the Bleached-IRL had mild to moderate inflammation (p<0.05). At the 30-days mark, there was no inflammation, but tertiary dentine formation in the bleached groups. Regarding IL-23, severe immunolabeling was observed in the Bleached group (p<0.05) at the 2-days mark; at the 30-days mark, there was a reduction in immunolabeling, in which the Bleached group had moderate and the Bleached-IRL group had mild immunolabeling (p>0.05). HIF-1α was more evident at the 2-days mark in the Bleached group, without significant difference with the Bleached-IRL (p>0.05). The difference was observed between the bleached and control groups, without immunolabeling (p<0.05); at the 30-days mark, the Bleached group had reduction in HIF-1α immunolabeling, while the Bleached-IRL had an increase; the difference remained between the bleached and the controls groups (p<0.05). Conclusion: Photobiomodulation using IRL minimized the inflammation and IL-23 immunolabeling in the pulp tissue of rats after dental bleaching, but did not influence significantly the HIF-1α immunolabeling.
Databáze:	MEDLINE
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