| Autor: |
Mahmoud AH; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., Mahmoud BK; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., Samy MN; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., Fouad MA; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., Kamel MS; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., Matsunami K; Department of Pharmacognosy, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. |
| Abstrakt: |
The bioactivity-guided fractionation of the total ethanolic extract of the leaves of Tabebuia aurea revealed the cytotoxic and antileishmanial potency of the ethyl acetate fraction, in which its phytochemical investigation resulted in the isolation of five triterpenes; identified as oleanolic acid ( 1 ), ursolic acid ( 2 ), pomolic acid ( 3 ), tormentic acid ( 4 ), 3 β ,6 β ,19 α -trihydroxy-urs-12-en-28-oic acid ( 5 ) in addition to one triterpenoid glucoside, spathodic acid 28- O - β -D-glucopyranoside ( 6 ). Whereas compound 1 showed cytotoxic activity against three different cell lines; A549, MCF-7 and HepG2 with IC 50 values of 31.7 ± 1.2, 27.4 ± 1.8 and 28.8 ± 1.1 μg/mL, respectively (etoposide as a positive control: 28.1 ± 4.2, 22.5 ± 4.5, and 20.4 ± 0.8 μg/mL, respectively), while compounds 1 and 2 showed antileishmanial activity with IC 50 values of 10.2 ± 0.9 μg/mL and 5.1 ± 0.4 μg/mL, respectively (miltefosine: 7.7 ± 2.1 μg/mL). |
