Novel LNA probe-based assay for the A1 and A2 identification of β-casein gene in milk samples.

Autor:	Giglioti R; Instituto de Zootecnia, Rua Heitor Penteado, n. 56, Nova Odessa, São Paulo 13380-011, Brazil., Hiromi Okino C; Embrapa Pecuária Sudeste, São Carlos, São Paulo, Brazil., Tainá Azevedo B; Instituto de Zootecnia, Rua Heitor Penteado, n. 56, Nova Odessa, São Paulo 13380-011, Brazil., Gutmanis G; Instituto de Zootecnia, Rua Heitor Penteado, n. 56, Nova Odessa, São Paulo 13380-011, Brazil., Morita Katiki L; Instituto de Zootecnia, Rua Heitor Penteado, n. 56, Nova Odessa, São Paulo 13380-011, Brazil., Cristina de Sena Oliveira M; Embrapa Pecuária Sudeste, São Carlos, São Paulo, Brazil., Eugênio Vercesi Filho A; Instituto de Zootecnia, Rua Heitor Penteado, n. 56, Nova Odessa, São Paulo 13380-011, Brazil.
Jazyk:	angličtina
Zdroj:	Food chemistry. Molecular sciences [Food Chem (Oxf)] 2021 Nov 28; Vol. 3, pp. 100055. Date of Electronic Publication: 2021 Nov 28 (Print Publication: 2021).
DOI:	10.1016/j.fochms.2021.100055
Abstrakt:	The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of β -casein gene ( CSN2 ) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe ( A1 and A2 ) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s).)
Databáze:	MEDLINE
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