Femtosecond laser oocyte enucleation as a low-invasive and effective method of recipient cytoplast preparation.
| Autor: | Osychenko AA; N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences. 4 Kosygina Street, Building 1, 119991 Moscow, Russia., Zalessky AD; N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences. 4 Kosygina Street, Building 1, 119991 Moscow, Russia., Tochilo UA; N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences. 4 Kosygina Street, Building 1, 119991 Moscow, Russia., Martirosyan DY; N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences. 4 Kosygina Street, Building 1, 119991 Moscow, Russia., Silaeva YY; Institute of Gene Biology Russian Academy of Sciences. 34/5 Vavilova Street, 119334 Moscow, Russia., Nadtochenko VA; N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences. 4 Kosygina Street, Building 1, 119991 Moscow, Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Biomedical optics express [Biomed Opt Express] 2022 Feb 14; Vol. 13 (3), pp. 1447-1456. Date of Electronic Publication: 2022 Feb 14 (Print Publication: 2022). |
| DOI: | 10.1364/BOE.449523 |
| Abstrakt: | Recipient cytoplast preparation, commonly performed by DNA aspiration with a needle, inevitably leads to the loss of reprogramming factors. As an alternative to the traditional enucleation technique, femtosecond laser enucleation can eliminate DNA effectively without loss of reprogramming factors and without oocyte puncturing. In this work we have performed oocyte enucleation by destructing the metaphase plate using a 795 nm femtosecond laser. The disability of the enucleated oocytes to develop after the parthenogenetic activation, as well as the lack of DNA staining luminescence, strongly confirms the efficiency of the femtosecond laser enucleation. The parthenogenetic development of oocytes after the cytoplasm treatment suggests a low-invasive effect of the laser enucleation technique. Competing Interests: The authors declare no conflicts of interest. (© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.) |
| Databáze: | MEDLINE |
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