Sustained endocrine and exocrine function in the pancreas of the Pacific spiny dogfish post-feeding.

Autor: Weinrauch AM; Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. Alyssa.Weinrauch@umanitoba.ca.; Bamfield Marine Sciences Centre, Bamfield, BC, V0R 1B0, Canada. Alyssa.Weinrauch@umanitoba.ca., Fehrmann F; Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada., Anderson WG; Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.; Bamfield Marine Sciences Centre, Bamfield, BC, V0R 1B0, Canada.
Jazyk: angličtina
Zdroj: Fish physiology and biochemistry [Fish Physiol Biochem] 2022 Jun; Vol. 48 (3), pp. 645-657. Date of Electronic Publication: 2022 Apr 12.
DOI: 10.1007/s10695-022-01070-8
Abstrakt: Secretions of the exocrine pancreas contain digestive enzymes integral to the digestive process. The Pacific spiny dogfish (Squalus suckleyi) has a discrete pancreas, divided into two lobes termed the dorsal and ventral lobes. These lobes drain into the anterior intestine via a common duct to enable digestion. Previous studies have identified that the exocrine pancreas produces (co)lipases, chymotrypsin, carboxypeptidase, and low levels of chitinases; however, investigations into other digestive enzymes are limited. We detect the presence of lipase, trypsin, and carbohydrase and show that activities are equivalent between both lobes of the pancreas. Additionally, we sought to investigate the influence of a single feeding event (2% body weight ration of herring by gavage) on enzyme activities over an extended time course (0, 20, 48, 72, 168 h) post-feeding. The results indicate that there are no differences in pancreatic tissue digestive enzyme activities between fed or fasted states. Analysis of acinar cell circumference post-feeding demonstrates a significant increase at 20 and 48 h, that returns to fasting levels by 72 h. No significant changes were observed regarding whole-tissue insulin or glucagon mRNA abundance or with glucose transporter (glut) 1 or 3. Yet, a significant and transient decrease in glut4 and sodium glucose-linked transporter mRNA abundance was found at 48 h post-feeding. We propose that the constant enzyme activity across this relatively large organ, in combination with a relatively slow rate of digestion leads to an evenly distributed, sustained release of digestive enzymes regardless of digestive state.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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