Tolerance of Human Fibroblasts to Benfo-Oxythiamine In Vitro.

Autor:	Yan M; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.; Department of Oral and Maxillofacial Surgery, Guiyang Hospital of Stomatology, Guiyang 050017, China., Smeets R; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.; Department of Oral and Maxillofacial Surgery, Division of 'Regenerative Orofacial Medicine', University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany., Gosau M; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany., Vollkommer T; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany., Fuest S; Department of Oral and Maxillofacial Surgery, Division of 'Regenerative Orofacial Medicine', University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany., Stetzer E; Benfovir AG, 64293 Darmstadt, Germany., Kluwe L; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany., Coy JF; Benfovir AG, 64293 Darmstadt, Germany., Burg S; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Jazyk:	angličtina
Zdroj:	International journal of environmental research and public health [Int J Environ Res Public Health] 2022 Mar 30; Vol. 19 (7). Date of Electronic Publication: 2022 Mar 30.
DOI:	10.3390/ijerph19074112
Abstrakt:	Objectives: To explore the potential application of B-OT in the aspiration tract. Materials and Methods: We conceived and optimized an in vitro model simulating the mouth-washing process to assess tolerance to B-OT on primary human gingival fibroblasts. Cells derived from 4 unrelated donors were flushed with medium containing drugs of various concentration for one minute twice daily for 3 days. Results: No effect was seen on the cells up to 1000 µM B-OT. In addition, we treated the cells with B-OT permanently in medium, corresponding to a systemic treatment. No effect was seen by 10 µM B-OT and only a slight reduction (approximately 10%) was seen by 100 µM B-OT. Conclusions: Our results suggest good tolerance of oral cells for B-OT, favoring the further development of this antiviral reagent as a mouth-washing solution and nasal spray.
Databáze:	MEDLINE
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