| Autor: |
Padmathilake KRE; Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada., Fernando WGD; Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular sciences [Int J Mol Sci] 2022 Apr 02; Vol. 23 (7). Date of Electronic Publication: 2022 Apr 02. |
| DOI: |
10.3390/ijms23073964 |
| Abstrakt: |
Leptosphaeria maculans causes blackleg disease, which is one of the most destructive diseases of canola ( Brassica napus L.). Due to the erosion of the current resistance in B. napus , it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of Rlm7 gene as resistance to its corresponding avirulence AvrLm7 gene is abundant. The Rlm7 line was inoculated with L. maculans isolate with AvrLm7 ; UMAvr7; and the CRISPR/Cas9 knockout AvrLm7 mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector proteins after 7-dpi in compatible interactions but not in incompatible interaction-confirmed that the pathogen was actively virulent only in compatible interactions. Salicyclic and jasmonic acid biosynthesis and signaling-related genes, defense-related PR1 gene (GSBRNA2T00150001001), and GSBRNA2T00068522001 in the NLR gene family were upregulated starting as early as 1- and 3-dpi in the incompatible interaction and the high upregulation of those genes after 7-dpi in compatible interactions confirmed the early recognition of the pathogen by the host and control it by early activation of host defense mechanisms in the incompatible interaction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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