Bothrops leucurus snake venom protein profile, isolation and biological characterization of its major toxin PLA 2 s-like.

Autor: Dias ÊR; Graduate Program in Biotechnology, State University of Feira de Santana (UEFS), Feira de Santana, BA, Brazil., de Oliveira LA; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil., Lauria PSS; Laboratory of Pharmacology and Experimental Therapeutics, College of Pharmacy, Federal University of Bahia, Salvador, BA, Brazil., Bordon KCF; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil., Rodrigues Domênico AM; Specialization in Diagnostic and Therapeutic Innovations, Hospital das Clínicas, São Paulo State University (UNESP), Botucatu, SP, Brazil., da Silva Guerreiro ML; Laboratory of Venomous Animals and Herpetology, State University of Feira de Santana, Feira de Santana, BA, Brazil., Wiezel GA; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil., Cardoso IA; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil., Rossini BC; Biotechnology Institute (IBTEC), São Paulo State University (UNESP), Botucatu, SP, Brazil; Department of Biological and Chemical Sciences, São Paulo State University (UNESP), Botucatu, SP, Brazil., Marino CL; Biotechnology Institute (IBTEC), São Paulo State University (UNESP), Botucatu, SP, Brazil; Department of Biological and Chemical Sciences, São Paulo State University (UNESP), Botucatu, SP, Brazil., Pimenta DC; Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, SP, Brazil., Arantes EC; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil., Casais-E-Silva LL; Laboratory of Neuroimmunoendocrinology and Toxinology, Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil., Branco A; Graduate Program in Biotechnology, State University of Feira de Santana (UEFS), Feira de Santana, BA, Brazil; Health Department, State University of Feira de Santana, Feira de Santana, BA, Brazil., Dos Santos LD; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil; Specialization in Diagnostic and Therapeutic Innovations, Hospital das Clínicas, São Paulo State University (UNESP), Botucatu, SP, Brazil; Biotechnology Institute (IBTEC), São Paulo State University (UNESP), Botucatu, SP, Brazil; Graduate Program in Graduate Program in Research and Development (Medical Biotechnology), Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil. Electronic address: lucilene.delazari@unesp.br., Biondi I; Laboratory of Venomous Animals and Herpetology, State University of Feira de Santana, Feira de Santana, BA, Brazil. Electronic address: ibiondi@uefs.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2022 Jul 15; Vol. 213, pp. 27-42. Date of Electronic Publication: 2022 Apr 08.
DOI: 10.1016/j.toxicon.2022.04.002
Abstrakt: Bothrops leucurus is considered as a snake of medical interest in the State of Bahia, Brazil. However, so far, there are no studies that provide a refined mapping of the composition of this venom. The aim of this work was to better understand the protein composition of B. leucurus snake venom and to isolate and biologically characterize the most abundant toxin, a basic PLA 2 -like. Shotgun proteomics approach identified 137 protein hits in B. leucurus venom subdivided into 19 protein families. The new basic PLA 2- like toxin identified was denominated Bleu-PLA 2 -like, it and other proteoforms represents about 25% of the total proteins in the venom of B. leucurus and induces myotoxicity, inflammation and muscle damage. Immunoreactivity assays demonstrated that B. leucurus venom is moderately recognized by bothropic and crotalic antivenoms, and on the other hand, Bleu-PLA 2 -like and its proteoforms are poorly recognized. Our findings open doors for future studies in order to assess the systemic effects caused by this snake venom in order to better understand the toxinological implications of this envenomation and, consequently, to assist in the clinical treatment of victims.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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