| Autor: |
Luo B; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China., Zhou J; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China., Li Z; Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China., Song J; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China., An P; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China., Zhang H; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China., Chen Y; Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China., Lan F; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China., Ying B; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China., Wu Y; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China. |
| Abstrakt: |
DNA methylation analysis holds great promise in the whole process management of cancer early screening, diagnosis, and prognosis monitoring. Nevertheless, accurate detection of target methylated DNA, especially its methylation ratio in the genome, remains challenging. Herein, we report for the first time an integrated strategy of target-induced nanoparticle-coupling and site-specific base oxidation damage for DNA methylation analysis with the assistance of well-designed nanosensors. The ultrahigh sensitivity for detecting target methylated DNA as low as 32 × 10 -17 M and high specificity for distinguishing 0.001% methylation ratio are achieved by this proposed strategy without amplification operations. Notably, the precise quantification of target DNA methylation ratio has been achieved for the first time. Through quantitative detection of target methylated DNA and methylation ratio, this proposed strategy could reliably diagnose and monitor cancer progression and treatment responses for colorectal cancer, which is superior to the clinical Septin 9 kit. It is anticipated that the proposed strategy has attractive application prospects in early diagnosis and monitoring for colorectal cancer and other various diseases. |
