Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans .

Autor:	Picciotto S; Cell-Tech HUB and Institute for Research and Biomedical Innovation, National Research Council of Italy (CNR), Palermo, Italy.; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy., Santonicola P; Institute of Biosciences and BioResources (IBBR)-National Research Council of Italy (CNR), Naples, Italy., Paterna A; Cell-Tech HUB and Institute of Biophysics, National Research Council of Italy (CNR), Palermo, Italy., Rao E; Cell-Tech HUB and Institute of Biophysics, National Research Council of Italy (CNR), Palermo, Italy., Raccosta S; Cell-Tech HUB and Institute of Biophysics, National Research Council of Italy (CNR), Palermo, Italy., Romancino DP; Cell-Tech HUB and Institute for Research and Biomedical Innovation, National Research Council of Italy (CNR), Palermo, Italy., Noto R; Cell-Tech HUB and Institute of Biophysics, National Research Council of Italy (CNR), Palermo, Italy., Touzet N; Centre for Environmental Research Innovation and Sustainability, Institute of Technology Sligo, Sligo, Ireland., Manno M; Cell-Tech HUB and Institute of Biophysics, National Research Council of Italy (CNR), Palermo, Italy., Di Schiavi E; Institute of Biosciences and BioResources (IBBR)-National Research Council of Italy (CNR), Naples, Italy., Bongiovanni A; Cell-Tech HUB and Institute for Research and Biomedical Innovation, National Research Council of Italy (CNR), Palermo, Italy., Adamo G; Cell-Tech HUB and Institute for Research and Biomedical Innovation, National Research Council of Italy (CNR), Palermo, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2022 Mar 24; Vol. 10, pp. 830189. Date of Electronic Publication: 2022 Mar 24 (Print Publication: 2022).
DOI:	10.3389/fbioe.2022.830189
Abstrakt:	Extracellular vesicles (EVs) are lipid membrane nano-sized vesicles secreted by various cell types for intercellular communication, found in all kingdoms of life. Nanoalgosomes are a subtype of EVs derived from microalgae with a sustainable biotechnological potential. To explore the uptake, distribution and persistence of nanoalgosomes in cells and living organisms, we separated them from a culture of the chlorophyte Tetraselmis chuii cells by tangential flow filtration (TFF), labelled them with different lipophilic dyes and characterized their biophysical attributes. Then we studied the cellular uptake of labelled nanoalgosomes in human cells and in C. elegans , demonstrating that they enter the cells through an energy dependent mechanism and are localized in the cytoplasm of specific cells, where they persist for days. Our data confirm that nanoalgosomes are actively uptaken in vitro by human cells and in vivo by C. elegans cells, supporting their exploitation as potential nanocarriers of bioactive compounds for theranostic applications. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Picciotto, Santonicola, Paterna, Rao, Raccosta, Romancino, Noto, Touzet, Manno, Di Schiavi, Bongiovanni and Adamo.)
Databáze:	MEDLINE
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