Clonal Diversity and Epidemiological Characteristics of ST239-MRSA Strains.

Autor:	Abd El-Hamid MI; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Sewid AH; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Samir M; Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Hegazy WAH; Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt., Bahnass MM; Department of Animal Medicine (Infectious Disease), Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Mosbah RA; Fellow Pharmacist, Zagazig University Hospital, Zagazig, Egypt., Ghaith DM; Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt., Khalifa E; Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt., Ramadan H; Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt., Alshareef WA; Department of Microbiology, Faculty of Pharmacy, October 6 University, 6th of October, Egypt., Alshareef HM; Department of Pharmacy Practice, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia., Ghoneim MM; Department of Pharmacy Practice, College of Pharmacy, Al Maarefa University, Ad Diriyah, Saudi Arabia., Al-Sanea MM; Pharmaceutical Chemistry Department, College of Pharmacy, Jouf University, Sakaka, Saudi Arabia., Bendary MM; Department of Microbiology and Immunology, Faculty of Pharmacy, Port Said University, Port Said, Egypt.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Mar 25; Vol. 12, pp. 782045. Date of Electronic Publication: 2022 Mar 25 (Print Publication: 2022).
DOI:	10.3389/fcimb.2022.782045
Abstrakt:	Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen associated with severe morbidity and mortality and poses a significant threat to public health worldwide. The genetic diversity based on sequence types of MRSA strains was illustrated in previous studies; meanwhile, the diversity along with the predominant sequence type, especially in Egypt, remains unknown. The purpose of the current study was to determine the diversity of the predominant MRSA clone ST239-MRSA (n = 50) isolated from different hosts and clinical samples and to illustrate the correlation between the resistance patterns, toxin genes, and the genetic background in Port-said and El-Sharkia Governorates, Egypt. The ST239-MRSA clone was analyzed by phenotypic antibiotyping and various genotypic assays comprising SCC mec , agr , spa , coa , and coa -RFLP in addition to toxin gene profiles. Most of the analyzed strains (40/50, 80%) were multidrug resistant (MDR), belonged to SCC mec -III, agr -I, and coa genotype I, and harbored sea and pvl genes. A negative correlation between the toxin gene profiles and antimicrobial resistance was recorded. Meanwhile, the correlation between the toxin gene profiles and the genetic background was not observed in this study. Although ST239-MRSA strains belonged to a single sequence type, they exhibited a high degree of phenotypic and genotypic diversity, indicating weak clonality and adaptability. With such diversity, it is assumed that these strains may have undergone different evolutionary processes during transmission events among and/or within a single host or tissue niche. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Abd El-Hamid, Sewid, Samir, Hegazy, Bahnass, Mosbah, Ghaith, Khalifa, Ramadan, Alshareef, Alshareef, Ghoneim, Al-Sanea and Bendary.)
Databáze:	MEDLINE
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