Small RNA-mediated regulation of the tet(M) resistance gene expression in Enterococcus faecium.

Autor: Le Neindre K; Unité Inserm U1230, Université de Rennes 1, Rennes, France; Service de Bactériologie-Hygiène hospitalière, CHU de Rennes, Rennes, France. Electronic address: killian.le-neindre@parisdescartes.fr., Dejoies L; Unité Inserm U1230, Université de Rennes 1, Rennes, France; Service de Bactériologie-Hygiène hospitalière, CHU de Rennes, Rennes, France. Electronic address: loren.dejoies@chu-rennes.fr., Reissier S; Unité Inserm U1230, Université de Rennes 1, Rennes, France. Electronic address: reissier.sophie@chu-amiens.fr., Guérin F; Service de Bactériologie-Hygiène hospitalière, CHU de Rennes, Rennes, France; CNR de la Résistance aux Antibiotiques (laboratoire associé 'Entérocoques'), Rennes, France. Electronic address: francois.guerin@chu-rennes.fr., Felden B; Unité Inserm U1230, Université de Rennes 1, Rennes, France. Electronic address: bfelden@univ-rennes1.fr., Cattoir V; Unité Inserm U1230, Université de Rennes 1, Rennes, France; Service de Bactériologie-Hygiène hospitalière, CHU de Rennes, Rennes, France; CNR de la Résistance aux Antibiotiques (laboratoire associé 'Entérocoques'), Rennes, France. Electronic address: vincent.cattoir@chu-rennes.fr.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2022 May-Jun; Vol. 173 (4-5), pp. 103941. Date of Electronic Publication: 2022 Apr 05.
DOI: 10.1016/j.resmic.2022.103941
Abstrakt: We investigated the role of a novel small RNA expressed in Enterococcus faecium (named Ern0030). We revealed that ern0030 was encoded within the 5'untranslated region of tet(M), a gene conferring tetracycline resistance through ribosomal protection. By RACE mapping, we accurately determined the boundaries of ern0030, which corresponded to Ptet. This upstream sequence of tet(M), Ptet, was previously described within transcriptional attenuation mechanism. Here, Northern blot analyses revealed three transcripts of different lengths (ca. 230, 150 and 100 nucleotides) expressed from Ptet. Phenotypically, the total deletion of ern0030 conferred a decrease in tetracycline MICs that was consistent with gene expression data showing no significant tet(M) induction under tetracycline SIC in ern0030-deleted mutant as opposed to a 10-fold increase of tet(M) expression in the wild-type strain. We investigated the transcriptional attenuation mechanism by toeprint assay. Whereas the expected tet(M) ribosome-binding site (RBS) was detected, the RBS of the putative leader peptide was not highlighted by toeprint assay, suggesting the transcriptional attenuation was unlikely. Here, we demonstrate that Ern0030 has a role in regulation of tet(M) expression and propose a novel model of tet(M) regulation alternative or complementary to transcriptional attenuation.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as a potential conflict of interest.
(Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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