Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo 19 F cardiovascular magnetic resonance in pigs.

Autor: Bönner F; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany. Florian.Boenner@med.uni-duesseldorf.de., Gastl M; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany., Nienhaus F; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany., Rothe M; Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research, Düsseldorf, Germany.; German Center for Diabetes Research, Partner, Düsseldorf, Germany., Jahn A; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany.; Central Animal Research Facility, Heinrich Heine University, Düsseldorf, Germany., Pfeiler S; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany., Gross U; Institute for Cardiac Diagnostics and Therapy (IKDT), Berlin, Germany., Schultheiss HP; Institute for Cardiac Diagnostics and Therapy (IKDT), Berlin, Germany., Ibanez B; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.; IIS-Fundación Jiménez Díaz Hospital, Madrid, Spain.; CIBERCV, Madrid, Spain., Kozerke S; Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland., Szendroedi J; Division of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany., Roden M; Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research, Düsseldorf, Germany.; German Center for Diabetes Research, Partner, Düsseldorf, Germany.; Division of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany., Westenfeld R; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany., Schrader J; Department of Molecular Cardiology, Heinrich Heine University, Düsseldorf, Germany.; Experimental Cardiovascular Imaging, Department of Molecular Cardiology, Heinrich Heine University, Düsseldorf, Germany., Flögel U; Department of Molecular Cardiology, Heinrich Heine University, Düsseldorf, Germany.; Cardiovascular Research Institute Düsseldorf (CARID), Medical Faculty, Heinrich Heine University, Düsseldorf, Germany., Heusch G; Institute for Pathophysiology, West German Heart and Vascular Center, University of Essen Medical School, Essen, Germany., Kelm M; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany.; Cardiovascular Research Institute Düsseldorf (CARID), Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: Basic research in cardiology [Basic Res Cardiol] 2022 Apr 07; Vol. 117 (1), pp. 21. Date of Electronic Publication: 2022 Apr 07.
DOI: 10.1007/s00395-022-00928-5
Abstrakt: Inflammatory cell infiltration is central to healing after acute myocardial infarction (AMI). The relation of regional inflammation to edema, infarct size (IS), microvascular obstruction (MVO), intramyocardial hemorrhage (IMH), and regional and global LV function is not clear. Here we noninvasively characterized regional inflammation and contractile function in reperfused AMI in pigs using fluorine ( 19 F) cardiovascular magnetic resonance (CMR). Adult anesthetized pigs underwent left anterior descending coronary artery instrumentation with either 90 min occlusion (n = 17) or without occlusion (sham, n = 5). After 3 days, in surviving animals a perfluorooctyl bromide nanoemulsion was infused intravenously to label monocytes/macrophages. At day 6, in vivo 1 H-CMR was performed with cine, T2 and T2* weighted imaging, T2 and T1 mapping, perfusion and late gadolinium enhancement followed by 19 F-CMR. Pigs were sacrificed for subsequent ex vivo scans and histology. Edema extent was 35 ± 8% and IS was 22 ± 6% of LV mass. Six of ten surviving AMI animals displayed both MVO and IMH (3.3 ± 1.6% and 1.9 ± 0.8% of LV mass). The 19 F signal, reflecting the presence and density of monocytes/macrophages, was consistently smaller than edema volume or IS and not apparent in remote areas. The 19 F signal-to-noise ratio (SNR) > 8 in the infarct border zone was associated with impaired remote systolic wall thickening. A whole heart value of 19 F integral ( 19 F SNR × milliliter) > 200 was related to initial LV remodeling independently of edema, IS, MVO, and IMH. Thus, 19 F-CMR quantitatively characterizes regional inflammation after AMI and its relation to edema, IS, MVO, IMH and regional and global LV function and remodeling.
(© 2022. The Author(s).)
Databáze: MEDLINE
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