ZOOMICS: Comparative Metabolomics of Red Blood Cells From Guinea Pigs, Humans, and Non-human Primates During Refrigerated Storage for Up to 42 Days.

Autor:	Bertolone L; Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States., Shin HKH; Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States., Baek JH; Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States., Gao Y; Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States., Spitalnik SL; Department of Pathology and Cell Biology, Columbia University, New York, NY, United States., Buehler PW; Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, United States.; Department of Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland School of Medicine, Baltimore, MD, United States., D'Alessandro A; Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States.; Department of Medicine, Division of Hematology, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2022 Mar 21; Vol. 13, pp. 845347. Date of Electronic Publication: 2022 Mar 21 (Print Publication: 2022).
DOI:	10.3389/fphys.2022.845347
Abstrakt:	Unlike other rodents, guinea pigs ( Cavia porcellus ) have evolutionarily lost their capacity to synthesize vitamin C (ascorbate) de novo and, like several non-human primates and humans, rely on dietary intake and glutathione-dependent recycling to cope with oxidant stress. This is particularly relevant in red blood cell physiology, and especially when modeling blood storage, which exacerbates erythrocyte oxidant stress. Herein we provide a comprehensive metabolomics analysis of fresh and stored guinea pig red blood cell concentrates ( n = 20), with weekly sampling from storage day 0 through 42. Results were compared to previously published ZOOMICS studies on red blood cells from three additional species with genetic loss of L-gulonolactone oxidase function, including humans ( n = 21), olive baboons ( n = 20), and rhesus macaques ( n = 20). While metabolic trends were comparable across all species, guinea pig red blood cells demonstrated accelerated alterations of the metabolic markers of the storage lesion that are consistent with oxidative stress. Compared to the other species, guinea pig red blood cells showed aberrant glycolysis, pentose phosphate pathway end product metabolites, purine breakdown products, methylation, glutaminolysis, and markers of membrane lipid remodeling. Consistently, guinea pig red blood cells demonstrated higher end storage hemolysis, and scanning electron microscopy confirmed a higher degree of morphological alterations of their red blood cells, as compared to the other species. Despite a genetic inability to produce ascorbate that is common to the species evaluated, guinea pig red blood cells demonstrate accelerated oxidant stress under standard storage conditions. These data may offer relevant insights into the basal and cold storage metabolism of red blood cells from species that cannot synthesize endogenous ascorbate. Competing Interests: AD’A is a founder of Omix Technologies Inc. AD’A is also a consultant for Altis Biosciences LLC., Rubius Inc., and Forma Inc. AD’A and SS are both consultants for Hemanext Inc. SS is also a consultant for Tioma, Inc., TCIP, Inc., and the Executive Director of the Worldwide Initiative for Rh Disease Eradication (WIRhE). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Bertolone, Shin, Baek, Gao, Spitalnik, Buehler and D’Alessandro.)
Databáze:	MEDLINE
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