Molecular epidemiology of Ascaris species recovered from humans and pigs in Cameroon.
| Autor: | Nkouayep VR; Research Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon., McManus DP; Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia., Mbida M; Research Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon., Gordon CA; Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia., Nejsum P; Department of Clinical Medicine, Faculty of Health, Aarhus University, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark. |
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| Jazyk: | angličtina |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene [Trans R Soc Trop Med Hyg] 2022 Oct 02; Vol. 116 (10), pp. 949-958. |
| DOI: | 10.1093/trstmh/trac020 |
| Abstrakt: | Background: In Cameroon, considerable research has been conducted on human ascariasis, but no studies have been undertaken to determine whether pigs contribute to the persistence of the infection in children or to unravel the evolutionary relationship between human-derived and pig-derived Ascaris. Methods: DNA was extracted from adult Ascaris worms collected from humans and pigs. Segments of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes were sequenced and analysed for 83 worms to dissect the local transmission dynamics of Ascaris in Cameroon. Results: The data showed high genetic diversity and revealed demographically expanding populations in the human and pig Ascaris samples. A restricted gene flow between Ascaris lumbricoides and Ascaris suum populations correlating with the preference for humans and pigs, respectively, as hosts was evident. Phylogenetic analyses and haplotype networks split the haplotypes into two major clusters, A and B. However, support for cross-transmission between hosts and hybridization were revealed through shared haplotypes among worms from both hosts. Conclusions: This study provides useful baseline information for future studies of the genetics of Ascaris in Cameroon and suggests that effective and sustainable control of human ascariasis should target both human and pig hosts. (© The Author(s) 2022. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene.) |
| Databáze: | MEDLINE |
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