Multiomic profiling of the acute stress response in the mouse hippocampus.

Autor: von Ziegler LM; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Floriou-Servou A; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Waag R; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Das Gupta RR; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.; Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland., Sturman O; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Gapp K; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Maat CA; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Kockmann T; Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Lin HY; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland.; Institute of Pharmacology and Toxicology, University of Zurich-Vetsuisse, Zurich, Switzerland., Duss SN; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Privitera M; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland., Hinte L; Laboratory of Nutrition and Metabolic Epigenetics, Institute of Food, Nutrition and Health, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland., von Meyenn F; Laboratory of Nutrition and Metabolic Epigenetics, Institute of Food, Nutrition and Health, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland., Zeilhofer HU; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland.; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.; Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland., Germain PL; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland.; Computational Neurogenomics, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland.; Laboratory of Statistical Bioinformatics, Department for Molecular Life Sciences, University of Zürich, Zurich, Switzerland., Bohacek J; Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland. johannes.bohacek@hest.ethz.ch.; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland. johannes.bohacek@hest.ethz.ch.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Apr 05; Vol. 13 (1), pp. 1824. Date of Electronic Publication: 2022 Apr 05.
DOI: 10.1038/s41467-022-29367-5
Abstrakt: The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. However, the underlying molecular cascades are unclear. Here, we use a brief swim exposure to trigger an acute stress response in mice, which transiently increases anxiety, without leading to lasting maladaptive changes. Using multiomic profiling, such as proteomics, phospho-proteomics, bulk mRNA-, single-nuclei mRNA-, small RNA-, and TRAP-sequencing, we characterize the acute stress-induced molecular events in the mouse hippocampus over time. Our results show the complexity and specificity of the response to acute stress, highlighting both the widespread changes in protein phosphorylation and gene transcription, and tightly regulated protein translation. The observed molecular events resolve efficiently within four hours after initiation of stress. We include an interactive app to explore the data, providing a molecular resource that can help us understand how acute stress impacts brain function in response to stress.
(© 2022. The Author(s).)
Databáze: MEDLINE
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