Inhibition of the Akt/NF-κB pathway is involved in the anti-gastritis effects of an ethanolic extract of the rhizome of Atractylodes macrocephala.
Autor: | Amin A; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: aftabamin@hkbu.edu.hk., Hossen MJ; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; Department of Animal Science, Patuakhali Science and Technology University, Dumki, Patuakhali, 8602, Bangladesh. Electronic address: jhossen@pstu.ac.bd., Fu XQ; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: makyfu@hkbu.edu.hk., Chou JY; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: choujiyao@gmail.com., Wu JY; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: 18481523@life.hkbu.edu.hk., Wang XQ; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: 18482759@life.hkbu.edu.hk., Chen YJ; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: saintchenyj@gmail.com., Wu Y; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: wuyingalicia@gmail.com., Yin CL; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: 16483529@life.hkbu.edu.hk., Dou XB; School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. Electronic address: xbdou77@163.com., Liang C; Division of Life Science, Center for Cancer Research and State Key Lab of Molecular Neuroscience, Hong Kong University of Science and Technology, Hong Kong, China; EnKang Pharmaceuticals, Limited, Guangzhou, China. Electronic address: bccliang@ust.hk., Chou GX; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address: chouguixin@shutcm.edu.cn., Yu ZL; Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; Research and Development Center for Natural Health Products, HKBU Institute for Research and Continuing Education, Shenzhen, China. Electronic address: zlyu@hkbu.edu.hk. |
---|---|
Jazyk: | angličtina |
Zdroj: | Journal of ethnopharmacology [J Ethnopharmacol] 2022 Jul 15; Vol. 293, pp. 115251. Date of Electronic Publication: 2022 Apr 02. |
DOI: | 10.1016/j.jep.2022.115251 |
Abstrakt: | Ethnopharmacological Relevance: Gastritis can lead to ulcers and the development of gastric cancer. The rhizome of Atractylodes macrocephala Koidz. (Asteraceae), a traditional Chinese medicinal herb, is prescribed for the treatment of gastric disorders, hepatitis and rheumatism. Its bio-active compounds are considered to be particularly effective in this regard. However, the molecular processes of the herb's anti-inflammatory activity remain obscure. This study elucidates a mechanism upon which an ethanolic extract of this herb (Am-EE) exerts anti-inflammation effects in RAW264.7 macrophage cells (RAW cells) stimulated by lipopolysaccharide (LPS) treatment and HCl Ethanol-stimulated gastritis rats. Aim of the Study: To investigate the anti-gastritis activities of Am-EE and explore the mode of action. Materials and Methods: Ethanol (95%) was used to prepare Am-EE. The quality of the extract was monitored by HPLC analysis. The in vivo effects of this extract were examined in an HCl Ethanol-stimulated gastritis rat model, while LPS-stimulated RAW cells were used for in vitro assays. Cell viability and nitric oxide (NO) production were observed by MTT and Griess assays. Real-time PCR was used to examine mRNA expression. The PGE Results: HPLC analysis indicates that Am-EE contains atractylenolide-1 (AT-1, 1.33%, w/w) and atractylenolide-2 (AT-2, 1.25%, w/w) (Additional Figure. A1). Gastric tissue damage (induced by HCl Ethanol) was significantly decreased in SD rats following intra-gastric application of 35 mg/kg Am-EE. Indistinguishable to the anti-inflammation effects of 35 mg/kg ranitidine (gastric medication). Am-EE treatment also reduced LPS-mediated nitric oxide (NO) and prostaglandin E Conclusion: Our study illustrates that Am-EE suppresses the Akt/IκBα/NF-κB pathway and exerts an anti-inflammatory effect. These novel conclusions provide a pharmacological basis for the clinical use of the A. macrocephala rhizome in the treatment and prevention of gastritis and gastric cancer. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
Externí odkaz: |