CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells.

Autor:	Ioannidis LJ; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia.; The Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia., Mitchell AJ; The Materials Characterisation and Fabrication Platform, Department of Chemical Engineering, The University of Melbourne, Parkville, VIC 3052, Australia., Zheng T; The Materials Characterisation and Fabrication Platform, Department of Chemical Engineering, The University of Melbourne, Parkville, VIC 3052, Australia., Hansen DS; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia.; The Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2022 Mar 30; Vol. 3 (2), pp. 101269. Date of Electronic Publication: 2022 Mar 30 (Print Publication: 2022).
DOI:	10.1016/j.xpro.2022.101269
Abstrakt:	High-dimensional mass cytometry provides unparalleled insight into the cellular composition of the immune system. Here, we describe a mass-cytometry-based protocol to examine memory CD4 + T cell and memory B cell (MBC) responses in human peripheral blood. This approach allows for the identification of >50 distinct memory CD4 + T cell and MBC populations from a single clinical sample. This highly reproducible protocol has been successfully applied to multiple infectious disease settings to identify correlates of susceptibility or protection from infection. For complete details on the use and execution of this protocol, please refer to Ioannidis et al. (2021). Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
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