| Autor: |
Ribieras AJ; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine., Ortiz YY; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine; Vascular Biology Institute, University of Miami Miller School of Medicine., Shrestha S; University of Miami., Huerta CT; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine., Shao H; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine; Vascular Biology Institute, University of Miami Miller School of Medicine., Boulina ME; Analytical Imaging Core Facility, University of Miami., Vazquez-Padron RI; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine; Vascular Biology Institute, University of Miami Miller School of Medicine., Liu ZJ; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine; Vascular Biology Institute, University of Miami Miller School of Medicine; zliu@med.miami.edu., Velazquez OC; Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine; Vascular Biology Institute, University of Miami Miller School of Medicine; ovelazquez@med.miami.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2022 Mar 16 (181). Date of Electronic Publication: 2022 Mar 16. |
| DOI: |
10.3791/63284 |
| Abstrakt: |
Peripheral arterial disease (PAD) is a significant cause of morbidity resulting from chronic exposure to atherosclerotic risk factors. Patients suffering from its most severe form, chronic limb-threatening ischemia (CLTI), face substantial impairments to daily living, including chronic pain, limited walking distance without pain, and nonhealing wounds. Preclinical models have been developed in various animals to study PAD, but mouse hindlimb ischemia remains the most widely used. There can be significant variation in response to ischemic insult in these models depending on the mouse strain used and the site, number, and means of arterial disruption. This protocol describes a unique method combining femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase (NOS) inhibitor to reliably induce footpad gangrene in Friend Virus B (FVB) mice that resembles the tissue loss of CLTI. While traditional means of assessing reperfusion such as laser Doppler perfusion imaging (LDPI) are still recommended, intracardiac perfusion of the lipophilic dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) is used to label the vasculature. Subsequent whole-mount confocal laser scanning microscopy allows for high-resolution, three-dimensional (3D) reconstruction of footpad vascular networks that complements traditional means of assessing reperfusion in hindlimb ischemia models. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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