Non-invasive in situ Visualization of the Murine Cranial Vasculature.

Autor: Rosenblum JS; Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States.; NeuroSimplicity LLC, Rockville, MD, 20852, United States., Cappadona AJ; Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States., Lookian PP; Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States., Chandrashekhar V; Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States.; NeuroSimplicity LLC, Rockville, MD, 20852, United States., Bryant JP; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, United States., Chandrashekhar V; NeuroSimplicity LLC, Rockville, MD, 20852, United States.; SmartSulis LLC, Trabuco Canyon, CA, 92679, United States., Zhao DY; Department of Neurosurgery, Medstar Georgetown University Hospital, Washington DC, 20007, United States., Knutsen RH; Laboratory of Vascular and Matrix Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, United States., Donahue DR; Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, United States., McGavern DB; Viral Immunology and Intravital Imaging Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, United States., Kozel B; Laboratory of Vascular and Matrix Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, United States., Heiss JD; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, United States., Zhuang Z; Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States.
Jazyk: angličtina
Zdroj: Cell reports methods [Cell Rep Methods] 2022 Jan 24; Vol. 2 (1).
DOI: 10.1016/j.crmeth.2021.100151
Abstrakt: Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels, iterative sample processing and micro-computed tomography, and automatic deformable image registration, feature extraction, and visualization. This methodology is applicable to any tissue and allows rapid exploration of normal and altered pathologic states.
Databáze: MEDLINE