A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms.
| Autor: | Tena-Garitaonaindia M; Department of Biochemistry and Molecular Biology II, Pharmacy School, University of Granada, Granada, Spain., Ceacero-Heras D; Department of Biochemistry and Molecular Biology II, Pharmacy School, University of Granada, Granada, Spain., Montoro MDMM; Clinical Analysis Service, Hospital Campus de la Salud, Granada, Spain.; Instituto de Investigación Biosanitaria (IBS), Granada, Spain., de Medina FS; Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Madrid, Spain., Martínez-Augustin O; Department of Biochemistry and Molecular Biology II, Pharmacy School, University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria (IBS), Granada, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Madrid, Spain.; Institute of Nutrition and Food Technology 'José Mataix,' Center of Biomedical Research, University of Granada, Granada, Spain., Daddaoua A; Department of Biochemistry and Molecular Biology II, Pharmacy School, University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria (IBS), Granada, Spain.; Institute of Nutrition and Food Technology 'José Mataix,' Center of Biomedical Research, University of Granada, Granada, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2022 Mar 16; Vol. 13, pp. 814448. Date of Electronic Publication: 2022 Mar 16 (Print Publication: 2022). |
| DOI: | 10.3389/fmicb.2022.814448 |
| Abstrakt: | The priority pathogen list of the World Health Organization classified Pseudomonas aeruginosa as the second top critical pathogen. Hence, the development of novel antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula edodes (AHCC ® ) on P. aeruginosa . AHCC ® was found to inhibit the growth rate and biofilm formation of strain PAO1. No change in swarming was observed, but AHCC ® hampered swimming and twitching motility. In accordance, a decreased expression of metabolism, growth, and biofilm formation genes was shown. AHCC ® also diminished the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10 and TNF by infected macrophages. This effect was related to a reduced phosphorylation of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCC ® has a potential role to prevent P. aeruginosa infections and may lead to the development of new therapies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Tena-Garitaonaindia, Ceacero-Heras, Montoro, de Medina, Martínez-Augustin and Daddaoua.) |
| Databáze: | MEDLINE |
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