Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live.

Autor: Slangen PLG; Department of Radiation Oncology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands., Porat Y; Novocure, Haifa, Israel., Mertz M; Bioimaging Facility, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands., van den Broek B; Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands., Jalink K; Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands., de Gooijer MC; Division of Pharmacology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands., van Tellingen O; Division of Pharmacology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands., Borst GR; Department of Radiation Oncology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.; The Christie NHS Foundation Trust, Wilmslow Road, M20 4BX Manchester, UK.; Division of Cancer Sciences, School of Medical Sciences, Faculty of Biology, Medicine & Health, The University of Manchester, 555 Wilmslow Road, M20 4GJ Manchester, UK.
Jazyk: angličtina
Zdroj: STAR protocols [STAR Protoc] 2022 Mar 30; Vol. 3 (2), pp. 101246. Date of Electronic Publication: 2022 Mar 30 (Print Publication: 2022).
DOI: 10.1016/j.xpro.2022.101246
Abstrakt: Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live TM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.
Competing Interests: Y.P. is an employee and shareholder of Novocure, which has applied for a patent for the use of a 'System for Viewing Cell Cultures under a Microscope Whilst Applying TTFields' (US20180202991A1).
(© 2022 The Authors.)
Databáze: MEDLINE
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