Induction of pancreatitis in mice with susceptibility to pancreatic cancer.

Autor:	Gitto SB; Ovarian Cancer Research Center, Division of Gynecology Oncology, Department of Obstetrics and Gynecology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA, United States; Department of Pathology and Laboratory Medicine, Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States., Nakkina SP; Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, United States., Beardsley JM; Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, United States., Parikh JG; Department of Pathology, Orlando VA Medical Center, Orlando, FL, United States., Altomare DA; Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, United States. Electronic address: deborah.altomare@ucf.edu.
Jazyk:	angličtina
Zdroj:	Methods in cell biology [Methods Cell Biol] 2022; Vol. 168, pp. 139-159. Date of Electronic Publication: 2022 Jan 21.
DOI:	10.1016/bs.mcb.2021.12.013
Abstrakt:	Chronic inflammation is known to be associated with pancreatic cancer, however a complete picture regarding how these pathologies intersect is still being characterized. In vivo model systems are critical for the study of mechanisms underlying how inflammation accelerates neoplasia. Repeat injection of cerulein, a cholecystokinin (CCK) analog, is widely used to experimentally induce acute and chronic pancreatitis in vivo. Chronic cerulein administration into genetically engineered mouse models (GEMMs) with predisposition to pancreatic cancer can induce a pro-inflammatory immune response, pancreatic acinar cell damage, pancreatic stellate cell activation, and accelerate the onset of neoplasia. Here we provide a detailed protocol and insights into using cerulein to induce pancreatitis in GEMMs, and methods to experimentally assess inflammation and pancreatic neoplasia. (Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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