Interaction of triblock copolymers (Pluronic®) with DMPC vesicles: a photophysical and computational study.

Autor: Calori IR; Department of Chemistry, Center of Nanotechnology and Tissue Engineering, Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14040-901, Brazil., Pinheiro L; Department of Chemistry, Research Nucleus of Photodynamic Therapy, State University of Maringá, Av. Colombo 5790, Maringá, Paraná 97020-900, Brazil., Braga G; Department of Chemistry, Research Nucleus of Photodynamic Therapy, State University of Maringá, Av. Colombo 5790, Maringá, Paraná 97020-900, Brazil., de Morais FAP; Department of Chemistry, Research Nucleus of Photodynamic Therapy, State University of Maringá, Av. Colombo 5790, Maringá, Paraná 97020-900, Brazil., Caetano W; Department of Chemistry, Research Nucleus of Photodynamic Therapy, State University of Maringá, Av. Colombo 5790, Maringá, Paraná 97020-900, Brazil., Tedesco AC; Department of Chemistry, Center of Nanotechnology and Tissue Engineering, Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14040-901, Brazil. Electronic address: atedesco@usp.br., Hioka N; Department of Chemistry, Research Nucleus of Photodynamic Therapy, State University of Maringá, Av. Colombo 5790, Maringá, Paraná 97020-900, Brazil.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2022 Jul 05; Vol. 275, pp. 121178. Date of Electronic Publication: 2022 Mar 19.
DOI: 10.1016/j.saa.2022.121178
Abstrakt: Pluronic/lipid mix promises stealth liposomes with long circulation time and long-term stability for pharmaceutical applications. However, the influence of Pluronics on several aspects of lipid membranes has not been fully elucidated. Herein it was described the effect of Pluronics on the structured water, alkyl chain conformation, and kinetic stability of dimyristoylphosphatidylcholine (DMPC) liposomes using interfacial and deeper fluorescent probes along with computational molecular modeling data. Interfacial water changed as a function of Pluronics' hydrophobicity with polypropylene oxide (PPO) anchoring the copolymers in the lipid bilayer. Pluronics with more than 30-40 PO units had facilitated penetration at the bilayer while shorter PPO favored a more interfacial interaction. Low Pluronic concentrations provided long-term stability of vesicles by steric effects of polyethylene oxide (PEO), but high amounts destabilized the vesicles as a sum of water-bridge cleavage at the polar head group and the reduced alkyl-alkyl interactions among the lipids. The high kinetic stability of Pluronic/DMPC vesicles is a proof-of-concept of its advantages and applicability in nanotechnology over conventional liposome-based pharmaceutical products for future biomedical applications.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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