Improved calibration of electrochemical aptamer-based sensors.

Autor: Downs AM; Department of Mechanical Engineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA.; Center for Bioengineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA., Gerson J; Department of Psychological and Brain Sciences, University of California Santa Barbara, Santa Barbara, CA, 93106, USA., Leung KK; Center for Bioengineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA.; Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA, 93106, USA., Honeywell KM; Department of Psychological and Brain Sciences, University of California Santa Barbara, Santa Barbara, CA, 93106, USA., Kippin T; Department of Psychological and Brain Sciences, University of California Santa Barbara, Santa Barbara, CA, 93106, USA.; The Neuroscience Research Institute and Department of Molecular Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, 93106, USA., Plaxco KW; Department of Mechanical Engineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA. kwp@ucsb.edu.; Center for Bioengineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA. kwp@ucsb.edu.; Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA, 93106, USA. kwp@ucsb.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Apr 01; Vol. 12 (1), pp. 5535. Date of Electronic Publication: 2022 Apr 01.
DOI: 10.1038/s41598-022-09070-7
Abstrakt: Electrochemical aptamer-based (EAB) sensors support the real-time, high frequency measurement of pharmaceuticals and metabolites in-situ in the living body, rendering them a potentially powerful technology for both research and clinical applications. Here we explore quantification using EAB sensors, examining the impact of media selection and temperature on measurement performance. Using freshly-collected, undiluted whole blood at body temperature as both our calibration and measurement conditions, we demonstrate accuracy of better than ± 10% for the measurement of our test bed drug, vancomycin. Comparing titrations collected at room and body temperature, we find that matching the temperature of calibration curve collection to the temperature used during measurements improves quantification by reducing differences in sensor gain and binding curve midpoint. We likewise find that, because blood age impacts the sensor response, calibrating in freshly collected blood can improve quantification. Finally, we demonstrate the use of non-blood proxy media to achieve calibration without the need to collect fresh whole blood.
(© 2022. The Author(s).)
Databáze: MEDLINE
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