Metabolomics based mechanistic insights to vasorelaxant and cardioprotective effect of ethanolic extract of Citrullus lanatus (Thunb.) Matsum. & Nakai. seeds in isoproterenol induced myocardial infraction.

Autor: Saqib F; Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan., Wahid M; Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan., Al-Huqail AA; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: aaalhuqail@pnu.edu.sa., Ahmedah HT; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Rabigh 25732, Saudi Arabia., Bigiu N; Faculty of Medicine, Transilvania University of Brasov, 500019 Brasov. Electronic address: nicusorbagiu@unitbv.ro., Irimie M; Faculty of Medicine, Transilvania University of Brasov, 500019 Brasov., Moga M; Faculty of Medicine, Transilvania University of Brasov, 500019 Brasov., Marc Vlaic RA; Food Engineering Department, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania. Electronic address: romina.vlaic@usamvcluj.ro., Pop OL; Department of Food Science, University of Agricultural Science and Veterinary Medicine, 400372 Cluj-Napoca, Romania., Chicea LM; Faculty of Medicine, 'Lucian Blaga' University of Sibiu,Romania.
Jazyk: angličtina
Zdroj: Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2022 Jun; Vol. 100, pp. 154069. Date of Electronic Publication: 2022 Mar 25.
DOI: 10.1016/j.phymed.2022.154069
Abstrakt: Background: Cardiovascular diseases (CVDs) are a significant cause of morbidity and death in the current world, posing a challenge to both developing and industrialized nation's health systems. Citrullus lanatus (Thunb.) Matsum. & Nakai. seeds have long been utilized to supplement and enhance health and treat cardiovascular illnesses. However, its treatments for CVDs are still unknown. More research is required to fully comprehend the impact of C. lanatus seeds on vasorelaxation and myocardial infractions.
Purpose: Therefore, an integrated metabolomics profiling technique was used to investigate possible pathways of C. lanatus in isoproterenol (ISO)-induced myocardial infarction (MI). Isoproterenol causes long-term cardiac hypertrophy by causing cardiomyocyte compensatory loss, eventually leading to heart failure.
Methods: In vitro models of vasoconstriction, atrium, and in vivo models of invasive blood pressure measurement and isoproterenol (ISO) induced cardiac hypertrophy in rats were used to understand underlying mechanistic by LC-MS/MS based dynamic metabolomics analysis of the serum and heart samples to be investigated the effect of ethanolic extract of C. lanatus (Cl.EtOH).
Results: Cl.EtOH exhibited vasorelaxant, negative chronotropic, and inotropic effects in in-vitro models whereas, a potent hypotensive effect was observed in normotensive rats. The Cl.EtOH protected the animals from ISO-induced myocardial infarction (MI) with therapeutic interventions in left ventricular thickness, cardiomyocyte hypertrophy, mRNA gene expression, biochemical assays, and metabolomic profiling of serum and heart tissues.
Conclusions: For the first time, our study confirmed that C. lanatus seeds (Cl.EtOH) possess significant antihypertensive and prevent ISO-induced myocardial infarction. These findings comprehensively demonstrated mechanistic insights of Cl.EtOH in vasorelaxation and myocardial infarction. The current study provides evidence for further mechanistic studies and the development of C. lanatus seeds as a potential therapeutic intervention for patients with cardiovascular disorders.
(Copyright © 2022 The Author(s). Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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