μMap-Red: Proximity Labeling by Red Light Photocatalysis.

Autor:	Buksh BF; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Knutson SD; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Oakley JV; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Bissonnette NB; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Oblinsky DG; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Schwoerer MP; Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States., Seath CP; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Geri JB; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Rodriguez-Rivera FP; Discovery Chemistry, Merck & Co., Kenilworth, New Jersey07033, United States., Parker DL; Discovery Chemistry, Merck & Co., Kenilworth, New Jersey07033, United States., Scholes GD; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States., Ploss A; Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States., MacMillan DWC; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2022 Apr 13; Vol. 144 (14), pp. 6154-6162. Date of Electronic Publication: 2022 Apr 01.
DOI:	10.1021/jacs.2c01384
Abstrakt:	Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments in animal models. Here, we report μMap-Red, a proximity labeling platform that uses a red-light-excited Sn IV chlorin e6 catalyst to activate a phenyl azide biotin probe. We validate μMap-Red by demonstrating photonically controlled protein labeling in vitro through several layers of tissue, and we then apply our platform in cellulo to label EGFR microenvironments and validate performance with STED microscopy and quantitative proteomics. Finally, to demonstrate labeling in a complex biological sample, we deploy μMap-Red in whole mouse blood to profile erythrocyte cell-surface proteins. This work represents a significant methodological advance toward light-based proximity labeling in complex tissue environments and animal models.
Databáze:	MEDLINE
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