The Isoflavanoid (+)-PTC Regulates Cell-Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line.

Autor: Moraes de Farias K; Programa de Pós-Graduação em Biotecnologia - RENORBIO - Rede Nordeste de Biotecnologia, Federal University of Ceará - UFC, 60020-181, Fortaleza, CE, Brazil.; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil., Rosa-Ribeiro R; Department of Structural and Functional Biology, Biology Institute, State University of Campinas, Campinas, 13083-970, SP, Brazil., Souza EE; Faculdade de Ciências Farmacêuticas, State University of Campinas, Campinas, 13083-859, SP, Brazil., Kobarg J; Faculdade de Ciências Farmacêuticas, State University of Campinas, Campinas, 13083-859, SP, Brazil., Banwell MG; Institute for Advanced and Applied Chemical Synthesis, Jinan University, Guangzhou, 510632, China., de Brito Vieira Neto J; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil., Leyenne Alves Sales S; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil., Roberto Ribeiro Costa P; Laboratório de Química Bioorgânica (LQB), Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, RJ, Brazil., Cavalcante Dos Santos R; Engenharia de Processos Químicos e Bioquímicos (EPQB), Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, RJ, Brazil., Vilaça Gaspar F; Laboratório de Química Bioorgânica (LQB), Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, RJ, Brazil., Gomes Barreto Junior A; Engenharia de Processos Químicos e Bioquímicos (EPQB), Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, RJ, Brazil., da Conceição Ferreira Oliveira M; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil., Odorico de Moraes M; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil., Libardi M Furtado C; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil.; Experimental Biology Center - NUBEX, University of Fortaleza, UNIFOR, Fortaleza, CE 60811-905, Brazil., Carvalho HF; Department of Structural and Functional Biology, Biology Institute, State University of Campinas, Campinas, 13083-970, SP, Brazil., Pessoa C; Programa de Pós-Graduação em Biotecnologia - RENORBIO - Rede Nordeste de Biotecnologia, Federal University of Ceará - UFC, 60020-181, Fortaleza, CE, Brazil.; Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM, Federal University of Ceará - UFC, Fortaleza, CE 60430-275, Brazil.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2022 May; Vol. 19 (5), pp. e202200102. Date of Electronic Publication: 2022 Apr 29.
DOI: 10.1002/cbdv.202200102
Abstrakt: Prostate cancer is the second most common malignancy in men and the development of effective therapeutic strategies remains challenging when more advanced, androgen-independent or insensitive forms are involved. Accordingly, we have evaluated, using flow cytometry, confocal microscopy and image analysis, the anti-proliferative effects of (+)-2,3,9-trimethoxypterocarpan [(+)-PTC, 1] on relevant human prostate cancer cells as well as its capacity to control mitosis within them. In particular, the studies reported herein reveal that (+)-PTC exerts anti-proliferative activity against the PC-3 cell lines by regulating cell-cycle progression with mitosis being arrested in the prophase or prometaphase. Furthermore, it emerges that treatment of the target cells with this compound results in the formation of monopolar spindles, disorganized centrosomes and extensively disrupted γ-tubulin distributions while centriole replication remains unaffected. Such effects suggest (+)-PTC should be considered as a possible therapy for androgen-insensitive/independent prostate cancer.
(© 2022 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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