Isolation of infectious Lloviu virus from Schreiber's bats in Hungary.

Autor: Kemenesi G; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary. kemenesi.gabor@gmail.com.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary. kemenesi.gabor@gmail.com., Tóth GE; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Mayora-Neto M; Viral Pseudotype Unit, Medway School of Pharmacy, Chatham Maritime, Universities of Kent & Greenwich, Kent, UK., Scott S; Viral Pseudotype Unit, Medway School of Pharmacy, Chatham Maritime, Universities of Kent & Greenwich, Kent, UK., Temperton N; Viral Pseudotype Unit, Medway School of Pharmacy, Chatham Maritime, Universities of Kent & Greenwich, Kent, UK., Wright E; Viral Pseudotype Unit, School of Life Sciences, University of Sussex, Falmer, Sussex, UK., Mühlberger E; Department of Microbiology, Boston University School of Medicine, Boston, MA, USA., Hume AJ; Department of Microbiology, Boston University School of Medicine, Boston, MA, USA., Suder EL; Department of Microbiology, Boston University School of Medicine, Boston, MA, USA., Zana B; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary., Boldogh SA; Aggtelek National Park Directorate, Jósvafő, Hungary., Görföl T; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary., Estók P; Department of Zoology, Eszterházy Károly University, Eger, Hungary., Szentiványi T; Institute of Ecology and Botany, ÖK Centre for Ecological Research, Vácrátót, Hungary., Lanszki Z; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Somogyi BA; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary., Nagy Á; Medical Centre, Hungarian Defence Forces, Budapest, Hungary., Pereszlényi CI; Medical Centre, Hungarian Defence Forces, Budapest, Hungary., Dudás G; Medical Centre, Hungarian Defence Forces, Budapest, Hungary., Földes F; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary., Kurucz K; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Madai M; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary., Zeghbib S; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary., Maes P; Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium., Vanmechelen B; Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium., Jakab F; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Mar 31; Vol. 13 (1), pp. 1706. Date of Electronic Publication: 2022 Mar 31.
DOI: 10.1038/s41467-022-29298-1
Abstrakt: Some filoviruses can be transmitted to humans by zoonotic spillover events from their natural host and filovirus outbreaks have occured with increasing frequency in the last years. The filovirus Lloviu virus (LLOV), was identified in 2002 in Schreiber's bats (Miniopterus schreibersii) in Spain and was subsequently detected in bats in Hungary. Here we isolate infectious LLOV from the blood of a live sampled Schreiber's bat in Hungary. The isolate is subsequently sequenced and cultured in the Miniopterus sp. kidney cell line SuBK12-08. It is furthermore able to infect monkey and human cells, suggesting that LLOV might have spillover potential. A multi-year surveillance of LLOV in bats in Hungary detects LLOV RNA in both deceased and live animals as well as in coupled ectoparasites from the families Nycteribiidae and Ixodidae. This correlates with LLOV seropositivity in sampled Schreiber's bats. Our data support the role of bats, specifically Miniopterus schreibersii as hosts for LLOV in Europe. We suggest that bat-associated parasites might play a role in the natural ecology of filoviruses in temperate climate regions compared to filoviruses in the tropics.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz: