Studying Protista WBR and Repair Using Physarum polycephalum.

Autor:	Sperry MM; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.; Department of Biology, Tufts University, Medford, MA, USA., Murugan NJ; Department of Biology, Algoma University, Sault Ste. Marie, ON, Canada. nirosha.murugan@algomau.ca.; Allen Discovery Center at Tufts University, Medford, MA, USA. nirosha.murugan@algomau.ca., Levin M; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA. michael.levin@tufts.edu.; Department of Biology, Tufts University, Medford, MA, USA. michael.levin@tufts.edu.; Allen Discovery Center at Tufts University, Medford, MA, USA. michael.levin@tufts.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2450, pp. 51-67.
DOI:	10.1007/978-1-0716-2172-1_3
Abstrakt:	Physarum polycephalum is a protist slime mould that exhibits a high degree of responsiveness to its environment through a complex network of tubes and cytoskeletal components that coordinate behavior across its unicellular, multinucleated body. Physarum has been used to study decision making, problem solving, and mechanosensation in aneural biological systems. The robust generative and repair capacities of Physarum also enable the study of whole-body regeneration within a relatively simple model system. Here we describe methods for growing, imaging, quantifying, and sampling Physarum that are adapted for investigating regeneration and repair. (© 2022. The Author(s).)
Databáze:	MEDLINE
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