A Prussian blue nanoparticles-based fluorescent nanoprobe for monitoring microRNA-92a and microRNA-21.

Autor: Li Y; Hunan Provincial Key Laboratory of Micro and Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, People's Republic of China., Guo R; Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China., Deng C; Hunan Provincial Key Laboratory of Micro and Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, People's Republic of China. dengchunyan@csu.edu.cn., Li D; National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China., Wu H; Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, 100850, People's Republic of China.
Jazyk: angličtina
Zdroj: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2022 Mar; Vol. 38 (3), pp. 497-504. Date of Electronic Publication: 2022 Apr 01.
DOI: 10.2116/analsci.20P455
Abstrakt: Since microRNA-92a (miR-92a) and microRNA-21 (miR-21) are crucial biomarkers for colorectal cancer (CRC), monitoring miR-92a and miR-21 in serum is very significant for the early diagnosis of CRC. In this work, we developed a simple and sensitive fluorescent biosensor for the detection of miR-92a and miR-21 based on the quenching ability of Prussian blue nanoparticles (PBNPs) to fluorophores. Carboxyl fluorescein (FAM)-modified ssDNA (P-92a) and Cyanine 5 (Cy5)-modified ssDNA (P-21) were completely complementary to miR-92a and miR-21 separately. They were adsorbed on PBNPs surface by the binding of PO 4 3- in DNA and Fe 3+ in PBNPs to fabricate the P-92a + P-21@PBNPs sensing system. The fluorescence responses from P-92a + P-21@PBNPs show good selection to miR-92a and a great linear process with the miR-92a concentration ranging from 1 to 30 nM (ΔF = 10.978 c miR-92a  + 71.457). Meanwhile, the fluorescence responses from P-92a + P-21@PBNPs is linearly relative to miR-21 from 3 to 30 nM; the linear equation is ΔF = 5.7560 c miR-21  + 48.729. Furthermore, the detections of miR-92a and miR-21 added in serum samples were achieved. In summary, this method is sensitive, highly specific, time-saving, cost-effective and applicable for the detection of miR-92a and miR-21. Therefore, this present sensor was expected to be used in clinical applications, which lays a potential foundation for an early diagnosis of cancer.
(© 2022. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.)
Databáze: MEDLINE
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