Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure.

Autor: Wunschik DS; Chemistry Department, Institute for Coatings and Surface Chemistry, Niederrhein University of Applied Sciences, Adlerstr. 32, 47798, Krefeld, Germany.; Deutsches Textilforschungszentrum Nord-West gGmbH, Adlerstr. 1, 47798, Krefeld, Germany.; Physical Chemistry and CENIDE (Center for Nanointegration), University Duisburg-Essen, Universitätsstraße 5, 45117, Essen, Germany., Lorenz A; Chemistry Department, Institute for Coatings and Surface Chemistry, Niederrhein University of Applied Sciences, Adlerstr. 32, 47798, Krefeld, Germany.; Physical Chemistry and CENIDE (Center for Nanointegration), University Duisburg-Essen, Universitätsstraße 5, 45117, Essen, Germany., Ingenbosch KN; Chemistry Department, Institute for Coatings and Surface Chemistry, Niederrhein University of Applied Sciences, Adlerstr. 32, 47798, Krefeld, Germany.; Deutsches Textilforschungszentrum Nord-West gGmbH, Adlerstr. 1, 47798, Krefeld, Germany.; Physical Chemistry and CENIDE (Center for Nanointegration), University Duisburg-Essen, Universitätsstraße 5, 45117, Essen, Germany., Gutmann JS; Deutsches Textilforschungszentrum Nord-West gGmbH, Adlerstr. 1, 47798, Krefeld, Germany.; Physical Chemistry and CENIDE (Center for Nanointegration), University Duisburg-Essen, Universitätsstraße 5, 45117, Essen, Germany., Hoffmann-Jacobsen K; Chemistry Department, Institute for Coatings and Surface Chemistry, Niederrhein University of Applied Sciences, Adlerstr. 32, 47798, Krefeld, Germany. kerstin.hoffmann-jacobsen@hs-niederrhein.de.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2022 Aug; Vol. 194 (8), pp. 3384-3399. Date of Electronic Publication: 2022 Mar 31.
DOI: 10.1007/s12010-022-03913-9
Abstrakt: A reusable support system for the immobilization of lipases is developed using hybrid polymer-inorganic core shell nanoparticles. The biocatalyst core consists of a silica nanoparticle. PMMA is grafted from the nanoparticle as polymer brush via ARGET ATRP (activator regenerated by electron transfer atom transfer radical polymerization), which allows defining the surface properties by chemical synthesis conditions. Lipase B from Candida antarctica is immobilized on the hybrid particles. The activity and stability of the biocatalyst are analyzed by spectroscopic activity analysis. It is shown that the hydrophobic PMMA brushes provide an activating surface for the lipase giving a higher specific activity than the enzyme in solution. Varying the surface structure from disordered to ordered polymer brushes reveals that the reusability of the biocatalyst is more effectively optimized by the surface structure than by the introduction of crosslinking with glutaraldehyde (GDA). The developed immobilization system is highly suitable for biocatalysis in non-native media which is shown by a transesterification assay in isopropyl alcohol and an esterification reaction in n-heptane.
(© 2022. The Author(s).)
Databáze: MEDLINE
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